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Characterization and Evaluation of Papayas Genetically Transformed with the Coat Protein Gene of Papaya Ringspot Virus

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Title:Characterization and Evaluation of Papayas Genetically Transformed with the Coat Protein Gene of Papaya Ringspot Virus
Authors:Lius, Suwenza
Date Issued:1994
Abstract:Genetically engineered papayas (Carica papaya L.) transformed with the coat protein gene of papaya ringspot virus (PRV) were characterized for morphology, fertility, transgene expression and evaluated for viral disease resistance. Among the R0 plants examined were 18 lines produced by means of particle bombardment and 4 lines produced by Agrobacterium transformation. Most of the plant lines appeared to be normal in morphology and fertility, except for a few that showed either a certain level of sterility or polyploidy.
Selected R1 progenies resulting either from self-pollinations of R0 plants or from crosses with non-transgenic plants, were evaluated in the greenhouse. Progeny analyses showed that most transgenes segregated in a simple Mendelian fashion. Progeny derived from R0 line 55-1 showed a high level of protection against PRV infection, while other R1 progenies showed variable responses, from delay of symptoms to complete susceptibility to the virus.
Two R0 lines and three of the coat protein positive (CP+) R1 lines were evaluated in the field for resistance to PRV. CP+ R0 line 55-1 and its subsequent R1 progeny showed a very good level of resistance to PRV, while other lines tested were either susceptible or showed only delayed symptoms following inoculation by the virus. The resistant line 55-1 showed vigorous growth with normal morphology and acceptable fruit quality under field conditions.
URI:http://hdl.handle.net/10125/56160
Appears in Collections: M.S. - Horticulture


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