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Towards the Development of a Recombinant Expression System for Yeast Reticulon Protein

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Item Summary

Title: Towards the Development of a Recombinant Expression System for Yeast Reticulon Protein
Authors: Kagawa, Allison
Keywords: Reticulon
Membrane protein
Mass spectrometry
Issue Date: Dec 2015
Publisher: [Honolulu] : [University of Hawaii at Manoa], [December 2015]
Abstract: Structure determination by X-ray diffraction requires large amounts of purified, functional protein making it especially challenging for membrane proteins due to their low abundance in the cell and instability outside the cell. In this study, we tested the results of our novel screening method for identifying the most promising membrane protein crystallization candidates from native cells through the recombinant expression of the yeast endoplasmic reticulum membrane protein, reticulon(RTN1). Using various mass spectrometry techniques, we were able to determine that RTN1 was not successfully expressed using an E. coli recombinant system, revealing a limitation in the application of our screening method for RTN1.
Description: M.S. University of Hawaii at Manoa 2015.
Includes bibliographical references.
Appears in Collections:M.S. - Chemistry

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