ScholarSpace will be down for maintenance on Thursday (8/16) at 8am HST (6pm UTC)
Please use this identifier to cite or link to this item:
Investigating Cryotherapy as a Novel Approach to Virus Elimination in Orchids
|2015-08-ms-ackerman_r.pdf||Version for non-UH users. Copying/Printing is not permitted||1.25 MB||Adobe PDF||View/Open|
|2015-08-ms-ackerman_uh.pdf||For UH users only||1.3 MB||Adobe PDF||View/Open|
|Title:||Investigating Cryotherapy as a Novel Approach to Virus Elimination in Orchids|
Cymbidium mosaic virus
Odontoglossum ringspot virus
show 1 moremicrotome
|Issue Date:||Aug 2015|
|Publisher:||[Honolulu] : [University of Hawaii at Manoa], [August 2015]|
|Abstract:||High quality orchids are important for Hawaii’s floriculture industry and a barrier to their production is viral infection, primarily from Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). Prevention and elimination techniques, including virus screening, thermotherapy, and chemotherapy, exist as solutions. Each approach offers varying levels of success; it is easier to eliminate CymMV than ORSV due to differential movement in orchids. Cryotherapy is a new approach to virus elimination in crops and ornamentals, using liquid nitrogen submergence of explants to burst virus-infected cells while preserving undifferentiated cells with cryo solutions. Vitrification and Droplet-vitrification methods were assessed to determine explant regeneration success. From this a modified Droplet-vitrification protocol was created for cryotherapy studies involving Dendrobium and Miltassia (CymMVinfected) and Oncidium (ORSV-infected). ORSV was not eliminated in the two regenerated explants following the protocol presented here. CymMV-infected explants failed to regenerate and could not be assessed for virus elimination.|
|Description:||M.S. University of Hawaii at Manoa 2015.|
Includes bibliographical references.
|Appears in Collections:||M.S. - Tropical Plant and Soil Sciences|
Please email firstname.lastname@example.org if you need this content in an ADA-compliant format.
Items in ScholarSpace are protected by copyright, with all rights reserved, unless otherwise indicated.