Please use this identifier to cite or link to this item: http://hdl.handle.net/10125/40610

Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria

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dc.contributor.author Kim, Youngchang
dc.contributor.author Joachimiak, Grazyna
dc.contributor.author Ye, Zi
dc.contributor.author Binkowski, T. Andrew
dc.contributor.author Zhang, Rongguang
dc.contributor.author Gornicki, Piotr
dc.contributor.author Callahan, Sean M.
dc.contributor.author Hess, Wolfgang R.
dc.contributor.author Haselkorn, Robert
dc.contributor.author Joachimiak, Andrzej
dc.date.accessioned 2016-05-05T02:06:16Z
dc.date.available 2016-05-05T02:06:16Z
dc.date.issued 2011-04
dc.identifier.uri http://hdl.handle.net/10125/40610
dc.description.abstract HetR is an essential regulator of heterocyst development in cyanobacteria. HetR binds to a DNA palindrome upstream of the hetP gene. We report the crystal structure of HetR from Fischerella at 3.0 Å. The protein is a dimer comprised of a central DNA-binding unit containing the N-terminal regions of the two subunits organized with two helix-turn-helix motifs; two globular flaps extending in opposite directions; and a hood over the central core formed from the C-terminal subdomains. The flaps and hood have no structural precedent in the protein database, therefore representing new folds. The structural assignments are supported by sitedirected mutagenesis and DNA-binding studies. We suggest that HetR serves as a scaffold for assembly of transcription components critical for heterocyst development.
dc.format.extent 6
dc.language.iso en-US
dc.publisher Proceedings of the National Academy of Sciences of the United States of America
dc.relation.uri http://www.pnas.org/content/108/25/10109.long
dc.subject Anabaena/Fischerella
dc.subject HTH motifs
dc.subject palindrome binding
dc.subject patterned development
dc.title Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria
dc.type Article
dc.type.dcmi Text
dc.identifier.doi 10.1073/pnas.1106840108
Appears in Collections: Department of Microbiology Faculty & Researcher Works


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