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Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria

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Item Summary Kim, Youngchang Joachimiak, Grazyna Ye, Zi Binkowski, T. Andrew Zhang, Rongguang Gornicki, Piotr Callahan, Sean M. Hess, Wolfgang R. Haselkorn, Robert Joachimiak, Andrzej 2016-05-05T02:06:16Z 2016-05-05T02:06:16Z 2011-04
dc.description.abstract HetR is an essential regulator of heterocyst development in cyanobacteria. HetR binds to a DNA palindrome upstream of the hetP gene. We report the crystal structure of HetR from Fischerella at 3.0 Å. The protein is a dimer comprised of a central DNA-binding unit containing the N-terminal regions of the two subunits organized with two helix-turn-helix motifs; two globular flaps extending in opposite directions; and a hood over the central core formed from the C-terminal subdomains. The flaps and hood have no structural precedent in the protein database, therefore representing new folds. The structural assignments are supported by sitedirected mutagenesis and DNA-binding studies. We suggest that HetR serves as a scaffold for assembly of transcription components critical for heterocyst development.
dc.format.extent 6
dc.language.iso en-US
dc.publisher Proceedings of the National Academy of Sciences of the United States of America
dc.subject Anabaena/Fischerella
dc.subject HTH motifs
dc.subject palindrome binding
dc.subject patterned development
dc.title Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria
dc.type Article
dc.type.dcmi Text
dc.identifier.doi 10.1073/pnas.1106840108
Appears in Collections: Department of Microbiology Faculty & Researcher Works

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