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Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria
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Item Summary
Title: | Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria |
Authors: | Kim, Youngchang Joachimiak, Grazyna Ye, Zi Binkowski, T. Andrew Zhang, Rongguang show 5 moreGornicki, Piotr Callahan, Sean M. Hess, Wolfgang R. Haselkorn, Robert Joachimiak, Andrzej show less |
Keywords: | Anabaena/Fischerella HTH motifs palindrome binding patterned development |
Issue Date: | Apr 2011 |
Publisher: | Proceedings of the National Academy of Sciences of the United States of America |
Related To: | http://www.pnas.org/content/108/25/10109.long |
Abstract: | HetR is an essential regulator of heterocyst development in cyanobacteria. HetR binds to a DNA palindrome upstream of the hetP gene. We report the crystal structure of HetR from Fischerella at 3.0 Å. The protein is a dimer comprised of a central DNA-binding unit containing the N-terminal regions of the two subunits organized with two helix-turn-helix motifs; two globular flaps extending in opposite directions; and a hood over the central core formed from the C-terminal subdomains. The flaps and hood have no structural precedent in the protein database, therefore representing new folds. The structural assignments are supported by sitedirected mutagenesis and DNA-binding studies. We suggest that HetR serves as a scaffold for assembly of transcription components critical for heterocyst development. |
Pages/Duration: | 6 |
URI/DOI: | http://hdl.handle.net/10125/40610 |
DOI: | 10.1073/pnas.1106840108 |
Appears in Collections: | Department of Microbiology Faculty & Researcher Works |
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