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Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria

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Title: Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria
Authors: Kim, Youngchang
Joachimiak, Grazyna
Ye, Zi
Binkowski, T. Andrew
Zhang, Rongguang
show 5 moreGornicki, Piotr
Callahan, Sean M.
Hess, Wolfgang R.
Haselkorn, Robert
Joachimiak, Andrzej

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Keywords: Anabaena/Fischerella
HTH motifs
palindrome binding
patterned development
Issue Date: Apr 2011
Publisher: Proceedings of the National Academy of Sciences of the United States of America
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Abstract: HetR is an essential regulator of heterocyst development in cyanobacteria. HetR binds to a DNA palindrome upstream of the hetP gene. We report the crystal structure of HetR from Fischerella at 3.0 Å. The protein is a dimer comprised of a central DNA-binding unit containing the N-terminal regions of the two subunits organized with two helix-turn-helix motifs; two globular flaps extending in opposite directions; and a hood over the central core formed from the C-terminal subdomains. The flaps and hood have no structural precedent in the protein database, therefore representing new folds. The structural assignments are supported by sitedirected mutagenesis and DNA-binding studies. We suggest that HetR serves as a scaffold for assembly of transcription components critical for heterocyst development.
Pages/Duration: 6
DOI: 10.1073/pnas.1106840108
Appears in Collections:Department of Microbiology Faculty & Researcher Works

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