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Investigation of a 45-kb Fragment of the Drosophila melanogaster Genome that is Homologous to a Receptor Tyrosine Kinase (RTK) found in Mammals
|Title:||Investigation of a 45-kb Fragment of the Drosophila melanogaster Genome that is Homologous to a Receptor Tyrosine Kinase (RTK) found in Mammals|
|Authors:||Nishimoto, Kyra Anne|
|Issue Date:||15 Jan 2014|
|Publisher:||University of Hawaii at Manoa|
|Abstract:||A 45-kb fragment within a 200-kb contig of the genome of Drosophila melanogaster has been identified as having exons with homology to a specific receptor tyrosine kinase (RTK) in mammals. 1bis receptor tyrosine kinase is of the Tyrosine Kinase related to trk (TKT) subclass. This particular subclass of RTK has a Factor VIII-like extracellular domain. Based on putative intron/exon splice sites, we have identified three potential exons in this 45-kb fragment. In this study, head and embryonic cDNA libraries were screened using a polymerase chain reaction (PCR)-mediated approach in an attempt to verify and clone these exons. Primers were created for all three exons. Primers within exon 2 have allowed amplification and cloning of an expected 620-bp product. Efforts are ongoing to generate PCR products from exons 1 and 3 and products spanning these various exons. We are also screening a cDNA library using a riboprobe created from exon 2. Using this probe, we have determined through in situ hybridization that embryonic salivary glands express this gene. This observation is consistent with the role of other Drosophila receptor tyrosine kinases that are known to be involved in the development of tubular structures.|
|Pages/Duration:||iv, 42 pages|
|Rights:||All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.|
|Appears in Collections:||Honors Projects for Biology|
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