Development Of A Monoclonal Antibody Specific to Ceruloplasmin

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2014-01-15
Authors
Cheong, Hausen
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University of Hawaii at Manoa
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Ceruloplasmin (132 kDa) is a copper-binding protein which functions as an anti-oxidant in the blood. Monoclonal antibodies are antibodies specific to a single antigen. Antibody producing lymphocytes were fused with myelomas to generate hybridomas which both produce antibody and survive in vitro. A polyethylene glycol fusion procedure was used to fuse splenocytes from a ceruloplasmin immunized BALB/c mouse and murine myelomas (P3 cell line also of BALB/c origin). Thes hybridomas were screened with an enzyme-linked immunosorbent assay (ELISA) using commercially available ceruloplasmin. A single hybridoma cell line producing monoclonal antibody specific to ceruloplasmin, a “ceruloplasminoma,” was isolated in this manner. Spectrophotometric analysis confirmed the specificity of this monoclonal antibody in inhibiting the oxidase activity of ceruloplasmin.
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viii, 67 pages
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