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Development Of A Monoclonal Antibody Specific to Ceruloplasmin

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Title:Development Of A Monoclonal Antibody Specific to Ceruloplasmin
Authors:Cheong, Hausen
Contributors:Nakamura, Jeffrey (advisor)
Biology (department)
Date Issued:15 Jan 2014
Publisher:University of Hawaii at Manoa
Abstract:Ceruloplasmin (132 kDa) is a copper-binding protein which functions as an anti-oxidant in the blood. Monoclonal antibodies are antibodies specific to a single antigen. Antibody producing lymphocytes were fused with myelomas to generate hybridomas which both produce antibody and survive in vitro. A polyethylene glycol fusion procedure was used to fuse splenocytes from a ceruloplasmin immunized BALB/c mouse and murine myelomas (P3 cell line also of BALB/c origin). Thes hybridomas were screened with an enzyme-linked immunosorbent assay (ELISA) using commercially available ceruloplasmin. A single hybridoma cell line producing monoclonal antibody specific to ceruloplasmin, a “ceruloplasminoma,” was isolated in this manner. Spectrophotometric analysis confirmed the specificity of this monoclonal antibody in inhibiting the oxidase activity of ceruloplasmin.
Pages/Duration:viii, 67 pages
Rights:All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: Honors Projects for Biology

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