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Induction Kinetics Of Yeast Galactokinase

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Title:Induction Kinetics Of Yeast Galactokinase
Authors:Chandler, Merrill
Contributors:Adams, Bruce (advisor)
Biology (department)
Date Issued:15 Jan 2014
Publisher:University of Hawaii at Manoa
Abstract:The kinetics of induction of enzyme systems have been well defined in bacterial systems. It has been found in bacterial systems that there is a lag time of 3 to 4 minutes after addition of inducer, before the first appearance of enzyme. The half life of M-RNA in bacterial systems has been determined to be between l.4 and 2.5 minutes. The determinations of the half-lives of M-RNA in higher systems however, have yielded more varied values. The values have varied from 2.7 to 30 minutes. It was the object of this research to determine the half-life of galactokinase M-RNA in the yeast Saccharomyces cerevisiae, using methods similar to those of Lawther and Cooper 1973. The experiments were inconclusive due to large fluctuations in enzyme levels after millipore filtration.
Pages/Duration:v, 24 pages
Rights:All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: Honors Projects for Biology

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