The Effect Of Chloroquine And Pyrimethamine On The Growth Of Two Strains Of The Human Malaria Parasite, Plasmodium Falciparum, After Long-Term Continuous In Vitro Cultivation

Date
2014-01-15
Authors
Campbell, Lori
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University of Hawaii at Manoa
Abstract
Two strains of the human malaria parasite, Plasmodium falciparum, were maintained in Aotus trivirgatus by serial passages, subsequently cultured continuously·in-vitro, and then tested to determine their susceptibility to two antimalarial drugs, chloroquine and pyrimethamine. The Uganda-Palo Alto (FUP) strain, susceptible to chloroquine and resistant to pyrimethamine when maintained in-vivo, was found to be more susceptible to chloroquine and susceptible to pyrimethamine after continuous in-vitro cultivation for 652 days. The Vietnam-Oak Knoll (FVO) strain, resistant to chloroquine and susceptible to pyrimethamine when maintained in-vivo, was found to be susceptible to chloroquine and more susceptible to pyrimethamine after continuous in-vitro cultivation for 297 days. It was also found that after continuous in-vitro cultivation, the FUP and FVO strains showed similar sensitivities to comparable concentrations of both chloroquine and pyrimethamine, whereas prior to long term in-vitro cultivation, their responses to these two drugs were distinct.
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