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A Requirement for Molecular Oxygen in the V1 Photocleavage of Dynein Heavy Chains
|Title:||A Requirement for Molecular Oxygen in the V1 Photocleavage of Dynein Heavy Chains|
|Issue Date:||15 Jan 2014|
|Publisher:||University of Hawaii at Manoa|
|Abstract:||The a and b heavy chains of dynein from sea urchin sperm flagella undergo specific cleavage at the Vl site when irradiated with 365 nm UV light, MgATP, and vanadate. This specific photocleavage of dynein results in the scission of the a and fJ heavy chains of Hr 428,000 to form HUVl and LUVl cleavage peptides of Hx 228,000 and Hx 200, 000, respectively (Gibbons I. R., Lee-Eiford A., Mocz G., Phillipson C. A., Tang W. -J. Y., & Gibbons B. H. (1987) J. Biol. Chem. 262, 2780-2786). To investigate the effects of molecular oxygen on the cleavage reaction, two procedures for irradiation under anoxic conditions have been developed.|
|Rights:||All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.|
|Appears in Collections:||Honors Projects for Biology|
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