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Monoclonal Antibodies to Ciguatoxin and Related Polyethers
|Title:||Monoclonal Antibodies to Ciguatoxin and Related Polyethers|
|Issue Date:||15 Jan 2014|
|Publisher:||University of Hawaii at Manoa|
|Abstract:||Monoclonal antibodies to ciguatoxin (CTX) and okadaic acid (OA) have been prepared using hybridoma technology. Spleen cells from BALB/C mice immunized with CTX-HSA and OA-HSA conjugates were fused with a mouse myeloma, P3X63-Ag8.653. Hybrids designated 5C8 (MonoAb-CTX ) and 10C3 (MonoAb-OA) were selected for further studies. The reactivity of the antibodies produced by the two hybrids with CTX and OA were tested using an enzyme immunoassay. The cross-reactivity of the antibodies to other polyethers and ionophores, ionomycin and A23187, was also investigated.|
|Pages/Duration:||iv, 34 pages|
|Rights:||All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.|
|Appears in Collections:||Honors Projects for Biology|
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