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Item Description Brostrom, Kathleen A. en_US 2012-03-15T18:56:27Z 2012-03-15T18:56:27Z 2005-08 en_US
dc.identifier.citation Brostrom KA. 2005. Are fecal sterols a possible alternative indicator of human waste contamination in hawaiian recreational waters?. Honolulu (HI): Water Resources Research Center, University of Hawaii at Manoa. en_US
dc.description.abstract Many of Hawaii’s recreational streams and beaches contain high fecal indicator bacteria levels that are not indicative of sewage pollution. Instead, this pollution is due to environmental sources of fecal bacteria which reside and multiply in tropical soils. Current EPA fecal indicator bacteria are no longer representative of human fecal contamination in tropical waters. Fecal sterols have been used as chemical indicators of fecal pollution in many parts of the world. The primary sterol found in human feces is coprostanol. Detection and quantification of coprostanol and related sterols using GCMS analysis provides a fingerprint that can be used to characterize fecal contamination. The objective of this study was to assay for fecal sterols as an independent method to determine whether streams in Hawaii are contaminated with sewage. This method was applied to ambient streams, a stream recently contaminated by a sewage spill, and a stream suspected to be affected by a sewage line leak. The results of this study showed that some ambient streams in Hawaii contain high levels of fecal indicator bacteria, but low concentrations of coprostanol (<10 ng/L). A stream contaminated with sewage during a sewage spill event contained high concentrations of coprostanol (18,000 ng/L) in the first 24 hours after contamination, but this level dropped to ≤ 60 mg/L after 72 hours. A stream suspected to be contaminated with sewage contained significant levels of coprostanol (>1000 ng/L) when fecal indicators were also high, confirming a possible sewage line leak. This study demonstrated that coprostanol is a useful and independent measurement of sewage pollution. It is best used in conjunction with other fecal indicators and human fecal markers if confirmation of human fecal pollution is sought. en_US
dc.format.extent x + 136 pages en_US
dc.language.iso en-US en_US
dc.publisher Water Resources Research Center, University of Hawaii at Manoa en_US
dc.relation Theses for the degree of Master of Science (University of Hawaii at Manoa). Microbiology; no. 3973 en_US
dc.relation.ispartofseries WRRC Unedited Reports. en_US
dc.relation.ispartofseries 2005-05 en_US
dc.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner. en_US
dc.subject.lcsh Water--Pollution--Hawaii--Testing. en_US
dc.subject.lcsh Sterols--Testing. en_US
dc.title Are Fecal Sterols a Possible Alternative Indicator of Human Waste Contamination in Hawaiian Recreational Waters? en_US
dc.type Thesis en_US
dc.type.dcmi Text en_US
local.identifier.callnumber Q111 .H3 no.3973 en_US

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