Please use this identifier to cite or link to this item:

Diversity and biogeography of the unique, tropical phylum Placozoa

File Description Size Format  
HAWN Q111.H3 4338 uh.pdf Version for UH users 4.63 MB Adobe PDF View/Open
HAWN Q111.H3 4338 r.pdf Version for non-UH users. Copying/Printing is not permitted 4.63 MB Adobe PDF View/Open

Item Summary Ward, Jillian 2011-07-22T00:08:50Z 2011-07-22T00:08:50Z 2008
dc.description Thesis (M.S.)--University of Hawaii at Manoa, 2008.
dc.description Includes bibliographical references (leaves 64-67).
dc.description x, 67 leaves, bound 29 cm
dc.description.abstract Phylum Placozoa consists of only a single described species, Trichoplax adhaerens. Although organisms within the phylum are morphologically indistinct, recent molecular evidence indicates cryptic diversity. To date there are 8 described placozoan haplotypes found in tropical regions world wide. Presented below is a community analysis of 30 placozoan isolates obtained from Hawai'i, as well as 14 samples collected from Puerto Rico. It was found that at least 5 of the 11 described haplotypes are found in Hawai'i, while only 2 different haplotypes were obtained from the Puerto Rican samples. Two separate genetic loci were analyzed using DNA from the Hawai'ian isolates, the mitochondrial 16S rDNA and a more variable location on the mitochondrial genome that encompasses the cox2 gene as well as an intergenic spacer region. It was found that both loci exhibited low levels of intra-haplotype variation and moderate levels of inter-haplotype variation, however the 16S locus was better able to capture diversity because the average single nucleotide polymorphism per base pair rate (SNP/bp) is 0.188 compared to the cox SNP/bp rate of 0.095 on average. Preliminary work was also done to explore the placozoan life cycle. Samples of near-shore water (between five and 11 liters) were taken during each of two field collections from 2 locations on Oahu and filtered. DNA was extracted from the filter and placozoan 16S rDNA primers were used to amplify product from the extract Possible product was detected by gel electrophoresis, but more work must be done to conclude whether or not placozoans inhabit the water column and at what concentration. Placozoans are tractable, easily cultured organisms making them ideal for ecological, speciation, and evolution studies.
dc.language.iso en-US
dc.relation Theses for the degree of Master of Science (University of Hawaii at Manoa). Oceanography; no. 4338
dc.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.title Diversity and biogeography of the unique, tropical phylum Placozoa
dc.type Thesis
dc.type.dcmi Text
Appears in Collections: M.S. - Oceanography

Please email if you need this content in ADA-compliant format.

Items in ScholarSpace are protected by copyright, with all rights reserved, unless otherwise indicated.