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Detecting Leptospira in water : evaluation of a proposed method

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M.S.Q111.H3_4239 DEC 2007_uh.pdf Version for UH users 3.3 MB Adobe PDF View/Open
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Title:Detecting Leptospira in water : evaluation of a proposed method
Authors:Hawkins, Ilima R.
Date Issued:2007
Abstract:Leptospirosis is the most widespread zoonosis in the world, and its geographic distribution is spreading. The disease is caused by Leptospira, a pathogenic group of spirochetes transferred between a variety of maintenance and accidental hosts. Few management practices exist because little is known about leptospire ecology and there is no reliable method for environmental detection. The goal of this work is to evaluate a two part methodology that isolates and detects pathogenic Leptospira in water. The first study uses suspensions of Leptospira, visually quantified in cells per milliliter, to evaluate the transmittance of different filters. The second study evaluates the sensitivity of a PCR detection method under real-world conditions. Results indicate a 0.2µm nitrocellulose filter is the optimal choice for filtration, and microbial DNA can be consistently recovered from used filter surfaces. Additional research will be necessary before this method can be used for field testing.
Description:Thesis (M.S.)--University of Hawaii at Manoa, 2007.
Includes bibliographical references (leaves 55-58).
vii, 58 leaves, bound 29 cm
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: M.S. - Natural Resources and Environmental Managament

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