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Induction of catalase in the athiorhodaceae

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Title:Induction of catalase in the athiorhodaceae
Authors:Shanmugam, Keelnatham T.
Date Issued:1969
Abstract:Catalase induction was investigated in various organisms belonging to the family Athiorhodaceae. Catalase induction could be demonstrated only in Rhodopseudomonas spheroides and related locally-isolated organisms. Both RNA and protein synthesis were found to be necessary for the induced synthesis. Although H2O2, the added inducer, was decomposed in the first 5 min., m-RNA synthesis continued up to 30 min. In Rh. spheroides, maximum induction occurred during the early stationary phase, while in TL-1, TL-4 and Rps. D, the induction was during the logarithmic phase of growth. !h. spheroides alone excreted porphyrins into the medium during the stationary phase. Inhibition of porphyrin synthesis by 8-Hydroxy quinoline (4 x 10^-5M) also inhibited catalase induction. The induced synthesis of catalase has been interpreted to require porphyrin synthesis. Growing TL-4 at constant pH decreased the log phase level of induction. Inducibility under these conditions was maximum at pH 7.3. Growing either Rh~ spheroides or TL-4 at constant pH had no effect on the stationary level of induction. Induction of catalase was observed only in those organisms which can effect an oxygen dependent conversion of the carotenoid spheroidene to spheroidenone. Inhibition of this carotenoid conversion by inhibitors like diphenylamine (3 x 10^-4M) or acridine (5 x 10^-4M) also inhibited the catalase induction. Lower concentration (0.5 to 1.0 x 10^-4M) of the inhibitors had a stimulatory effect. The data is discussed in terms of the involvement of carotenoids in the induction of catalase.
Thesis (Ph. D.)--University of Hawaii, 1969.
Bibliography: leaves [84]-90.
vi, 90 l illus
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: Ph.D. - Microbiology

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