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Investigation of selenoprotein k function and associated proteins
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|Title:||Investigation of selenoprotein k function and associated proteins|
|Authors:||Fay, Jeffrey D.|
|Date Issued:||May 2012|
|Publisher:||[Honolulu] : [University of Hawaii at Manoa], [May 2012]|
|Abstract:||Selenoprotein K (SelK) is a small (16kD) single pass transmembrane protein localized to the ER. An Src Homology 3 (SH3)-binding domain within the amino acid sequence was exploited to identify potential protein-protein interactions. The effects of SelK deficiency in immune cells and effector calcium flux served as a model system to elucidate a function for SelK.|
Immunoprecipitation and mass spectrophotometry identification of potential binding partners returned Arf-GAP2 and pyruvate kinase as possible binding partners. Plasmid DNA vectors were designed for use in the Two-Hybrid system to confirm preliminary data of suspected interactions with the creation of fusion proteins coding for Vav1 or Vav2 SH3 domain and the SelK SH3 binding domain.
Protein-protein interaction could not be confirmed in this system however SelK seems to play a role in receptor mediated calcium flux, and subsequent low-level production of nitric oxide by way of neuronal nitric oxide synthase.
|Description:||M.S. University of Hawaii at Manoa 2012.|
Includes bibliographical references.
|Appears in Collections:||
M.S. - Cell and Molecular Biology|
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