Please use this identifier to cite or link to this item:

The role of selenoproteins in protection against methamphetamine-induced dopaminergic neurodegeneration

File Description Size Format  
Barayuga_Stephanie_r.pdf Version for non-UH users. Copying/Printing is not permitted 1.02 MB Adobe PDF View/Open
Barayuga_Stephanie_uh.pdf Version for UH users 1.14 MB Adobe PDF View/Open

Item Summary Barayuga, Stephanie Malbog 2015-10-02T20:59:23Z 2015-10-02T20:59:23Z 2014-05
dc.description M.S. University of Hawaii at Manoa 2014.
dc.description Includes bibliographical references.
dc.description.abstract Methamphetamine (MA) increases oxidative stress through actions on dopaminergic transmission. Selenium (Se) is an antioxidant trace element that is necessary for normal brain functions. Previous studies have shown that Se protects against MA neurotoxicity and is necessary for proper dopamine function. The specific aim of the project is to determine the role of antioxidant selenoproteins, including the glutathione peroxidases (GPXs), in protecting dopaminergic neurons from MA toxicity in vitro and in vivo. In SH-SY5Y cells, MA decreased protein levels for GPX1 and GPX4. However, both proteins were upregulated with increasing Se concentration. GPX enzymatic activity was increased by Se concentrations and decreased by MA and correlated with GPX protein levels. MA reduced total intracellular GSH levels at lower Se concentrations, while the oxidized fraction of GSH was increased at higher Se levels. Extracellular GSH was increased in 0 nM Se only, with no changes from MA or any changes in the oxidized fraction of GSH. Additionally, MA increased ROS levels in cell cultures grown in 0 nM Se, but Se supplementation prevented the increase. Our in vivo results show that GPX1 and GPX4 increase with Se, whereas, DAT and TH expression do not appear to change. The results of our study indicate that a MA-induced reduction in GPX levels can contribute to increased oxidative stress. GPX reduction can be prevented increasing Se levels. These findings have important implications for treating patients with acute methamphetamine toxicity.
dc.language.iso eng
dc.publisher [Honolulu] : [University of Hawaii at Manoa], [May 2014]
dc.relation Theses for the degree of Master of Science (University of Hawaii at Manoa). Molecular Biosciences and Bioengineering.
dc.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.subject selenoproteins
dc.title The role of selenoproteins in protection against methamphetamine-induced dopaminergic neurodegeneration
dc.type Thesis
dc.type.dcmi Text
Appears in Collections: M.S. - Molecular Biosciences and Bioengineering

Please email if you need this content in ADA-compliant format.

Items in ScholarSpace are protected by copyright, with all rights reserved, unless otherwise indicated.