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Quantitative inheritance of immunological response in swine
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|Title:||Quantitative inheritance of immunological response in swine|
|Abstract:||Pigs were used as an animal model for studying the sources of variation in immunological response, under selected dose levels of immunization and at various stages during the development of immunity. Special emphasis was focused on the genetic component of the variation. Bovine serum albumin was selected as an antigen to inject pigs at the age of 28 days. Based on the preliminary trials, two dose levels of BSA were chosen, namely 5 and 50 mg. The kinetics of immune response were studied to decide the time for taking blood samples for the main study. Three blood samples were taken at 14, 21 and 42 days after the first immunization, each representing the primary response, Rp, secondary response, Rs, and peak response, Rm, respectively. A total of 802 pigs which were offspring of 18 purebred boars, each mated with six females, were used in the main study. Antigen binding capacity was used for measuring the antibody response. Data expressed as percent antigen bound was transformed by angle. No significant difference was found in the effect of sex, birth weight, weaning weight or pre-weaning daily gain on immune response. Pigs which received 5 mg of BSA gave consistently higher response than those receiving 50 mg of dose level in all three response measurements, i.e. Rp, Rs and Rm. The difference between the two dose levels and among litters in all responses were highly significant, but did not show any interaction between the dose level and litter. The intrarelationship of immune response measured at the three stages were found positively correlated. The difference among sires was found to be statistically significant in immune response under the dose of 5 mg of BSA except for the primary response. Variations among dams within sires were significant only in the primary response. For pigs treated with 50 mg of BSA, no sire difference in the peak response was detected at the 1% significant level but the difference existed at the 5% significant level. Significant difference was found among dams within sires. The genetic contribution to the early response could not be important. As variation of immune response in 50 mg of BSA would be expected to manifest mainly enviromnenta1 influences, this part of data was not used for estimation of genetic parameters. The genetic variance of the three immune responses, 29, 59 and 51 percent of the phenotypic variance, showed an important effect on the secondary and peak response.. The litter environment decreased in importance from 25% of the phenotypic variance in primary response to zero in the secondary and peak response. The individual environmental variance showed a stable variation among the three immune responses. The genetic correlations between the two adjacent stages of immune response, i.e. Rp and Rs, and Rs and Rm were considerably higher than that between Rp and Rm. Similar results were also found in the correlation measurements of individual environment and phenotypic measurement. The heritability obtained from sire and dam component combined for secondary and peak response were 0.51 ± 0.04 and 0.40 ± 0.04. The estimates were considered to be more precise than those calculated from other variance components, In conclusion, the mu1tigenic control of immune response was demonstrated in the present study.|
Thesis (Ph. D.)--University of Hawaii at Manoa, 1977.
Bibliography: leaves -92.
viii, 92 leaves ill
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|Appears in Collections:||Ph.D. - Genetics|