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Title: Genetic studies of the oriental fruit fly, Bactrocera dorsalis (Hendel), (Deptera: Tephritidae) : description and linkage analysis of genetic markers 
Author: McCombs, Susan Denise
Date: 1992
Abstract: The oriental fruit fly, Bactrocera dorsalis (Hendel), is an important agricultural pest in the Pacific Basin and a constant threat to the United States mainland. The extensive economic losses caused by this polyphagous species have been the impetus for research directed towards developing effective non-toxic control measures. A library of genetic markers was constructed by isolating morphological variants in a series of mutagenesis trials using ethyl methane sulfonate and formaldehyde, and by screening of laboratory and geographic populations. Twenty markers were described, eye color: cherry, copper, gold-grey, Grape, mandarin red, white eye, and yellow eye; eye structure: amethyst, Azure, Furrow, matte, and notched eye; puparium color: melanistic and white puparium; puparium morphology: elongate puparium and robust puparium; and wing structure: crossveinless, curled, notch wing and small wing. The mode of inheritance of marker genes was determined by reciprocal single pair crosses between mutant and wild-type flies. The F2 progeny numbers were analyzed by chi-square test for goodness of fit to proposed models for autosomal or sex-linkage, recessive or dominant inheritance. All genes were autosomal in nature. Furrow was inherited as a dominant gene, Grape and Azure as incompletely dominant genes and all others were inherited as recessive traits. F2 progeny numbers from reciprocal crosses with the marker genes in the repulsion configuration were analyzed to assign 15 markers to the five autosomal linkage groups. Map distances were determined from recombinant F2 progeny numbers from crosses in coupling. Total mapping units (m.u.) were equal to the mean recombination frequency times 100. The linear order of genes in linkage group A was given with mandarin red at position 0, robust puparium at 5.4 m.u., melanistic at 14.1 m.u., matte at 26.7 m.u., and elongate puparium at 30.2 m.u. Genes in linkage group C were mapped relative to the white puparium locus, amethyst at 23.4 m.u. from white puparium, gold-grey at 6.7 m.u. and white eye at 31.2 m.u. Grape and small wing in linkage group D were 43.2 m.u. apart. Yellow eye and notched eye were the only markers in linkage groups Band E, respectively. Five markers, Azure, curled, crossveinless, Furrow and notch wing remain unmapped.
Description: Thesis (Ph. D.)--University of Hawaii at Manoa, 1992. Includes bibliographical references (leaves 178-188) Microfiche. xxi, 188 leaves, bound ill. (some col.) 29 cm
URI: http://hdl.handle.net/10125/9775
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Keywords: Fruit-flies -- Genetics

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