Item Description

Show full item record

Title: Isolation of a sugarcane gene promoter and its application to chimeric gene expression in sugarcane protoplasts 
Author: Barry, Kelly
Date: 1992
Abstract: The promoter region of a sugarcane gene has been isolated and characterized from suspension culture cells of Saccharum ssp. hybrid H50-7209. The transcription initiation site was determined by primer extension and physical characteristics such as a 5' transcript splice site, a TATA element, and several upstream repetitive sequences were inferred by sequence analysis. The sugarcane promoter element was isolated by PCR amplification and fused to the GUS reporter gene coding sequence. This chimeric gene construct was transferred to sugarcane protoplasts by electroporation and transient gene expression was monitored by the GUS fluorimetric assay. The sugarcane promoter was approximately five times more effective than the CaMV 35S promoter at driving heterologous gene expression in sugarcane protoplasts.
Description: Thesis (Ph. D.)--University of Hawaii at Manoa, 1992. Includes bibliographical references (leaves 87-106) Microfiche. xii, 106 leaves, bound ill. 29 cm
URI: http://hdl.handle.net/10125/9471
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.

Item File(s)

Description Files Size Format View
Restricted for viewing only uhm_phd_9230472_r.pdf 3.228Mb PDF View/Open
For UH users only uhm_phd_9230472_uh.pdf 3.196Mb PDF View/Open

This item appears in the following Collection(s)

Search


Advanced Search

Browse

My Account

Statistics

About