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Title: A Simple Device for Making Successive Photomicrographic Records of Large Groups of Developing Organisms 
Author: Hsiao, Sidney C; Fujii, Walter K; Fine, Helen H
Date: 1963-07
Publisher: University of Hawai'i Press
Citation: Hsiao SC, Fujii WK, Fine HH. 1963. A simple device for making successive photomicrographic records of large groups of developing organisms. Pac Sci 17(3): 321-328.
Abstract: In our analysis of the effect of ionizing radiation
upon the cleavage of sea urchin zygotes
we found it necessary to take successive photomicrographs
of a large number of eggs, in
different samples, which had been exposed to
graded doses of ionizing radiation (Hsiao and
Daniel, 1960). In order to estimate the rate
of cleavage of the irradiated samples of fertilized
eggs it is highly desirable to follow the
cleavage of each egg in every sample and make
photomicrographic records for later analysis.
In other words, we need to take time-lapse
pictures of the developing eggs subjected to
different amounts of radiation so as to calculate
the rate of cleavage and correlate it with
dosage. After some preliminary trials we have
put together, using commonly available materials,
a simple device capable of taking photomicrographs
repeatedly from the same field
in a series of samples of irradiated eggs at
specified time intervals. By lining up, according
to time, prints made from each field, each
egg can be identified and its cleavage followed
from the first to the last frame in the series,
and its rate of cleavage can be calculated. It
occurs to us that investigators who have occasion
to record developmental and other recurrent
phenomena may find this simple device
useful. A brief description of its method of construction
and manipulation is reported in this
paper.
ISSN: 0030-8870
URI: http://hdl.handle.net/10125/7144

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