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Diversity and transport of bacteria and viruses in soils following irrigation with recycled water

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Item Summary

Title: Diversity and transport of bacteria and viruses in soils following irrigation with recycled water
Authors: Huang, Tieshi
Advisor: Babcock, Roger
Issue Date: Dec 2003
Publisher: University of Hawaii at Manoa
Abstract: Field and laboratory analyses were conducted to study bacteria and virus transport and diversity in surface soils following irrigation with recycled water. Field test plots were irrigated with R-1 (oxidized, filtered, disinfected) water and tap water, and pan lysimeters were used to collect leachate water samples. R-1, R-2 (oxidized, disinfected), R-3 (oxidized only) and tap water were applied to soil columns. Fecal coliform and coliphage were only found in the leachate from the R-3 soil column. Polymerase chain reaction denaturing gradient gel electrophoresis analysis (PCR-DGGE) of 165 rRNA genes was used to analyze the bacterial population in leachate and soil samples from different depths. The R-1 field test plot had a more diversified bacterial community than a tap water control plot. Surface soils from field test plots had more bacteria species than that of bottom soils. Different DGGE banding profiles were found at different depths in R-1, R-2 and R-3 effluent soil columns. UV dosage did have some effect on soil microbial diversity.
Description: ix, 42 leaves
URI/DOI: http://hdl.handle.net/10125/7033
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:M.S. - Civil Engineering



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