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dc.contributor.advisor Lozanoff, Scott en_US
dc.contributor.author Margaryan, Edith en_US
dc.date.accessioned 2009-03-06T19:31:26Z en_US
dc.date.available 2009-03-06T19:31:26Z en_US
dc.date.issued 2002-12 en_US
dc.identifier.uri http://hdl.handle.net/10125/6950 en_US
dc.description xiii, 56 leaves en_US
dc.description.abstract A newly identified mouse mutation, called Br, displays heritable phenotypic features of frontonasal dysplasia (FND) and multic0ystic renal hypodysplasia (MRHD). The Br mutation is mapped to distal murine chromosome 17, but the causative gene remains unknown. The objective of this project was to analyze expression of three positional candidate genes, Six3, Zfp161 and ALK, in their target tissues. Two of these genes, Six3 and the putative mouse TGIF homologue, Zfp161, are candidates for the neurodevelopmental disorder, holoprosencephaly (HPE). Previous reports indicate a possible pathogenic relationship between HPE and FND, implicating HPE candidate genes in development of FND. The third gene of interest, ALK, is a neural developmental gene, also involved in renal morphogenesis. Embryos were collected on gestational day (GD) 18, and brain and kidney tissues were extracted. RT-PCR experiments were performed and expression was visualized with ethidium bromide staining. All three genes were found to be expressed in all examined tissues. Expression of the Six3 gene in the kidney was not previously described in the literature. This implies a novel role of Six3 in the renal development. Detailed mutational analysis of identified Six3 expression will be useful for evaluating its role in Br mutation. en_US
dc.publisher University of Hawaii at Manoa en_US
dc.relation Theses for the degree of Master of Science (University of Hawaii at Manoa). Biomedical Sciences (Cell & Molecular Biology); no. 3747 en_US
dc.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner. en_US
dc.rights.uri https://scholarspace.manoa.hawaii.edu/handle/10125/864 en_US
dc.title Expression of SIX3, ZFP161 and ALK Genes in the Brain and Kidneys of3H1 Br Mutant Mice en_US
dc.type Thesis en_US
dc.type.dcmi Text en_US
dc.contributor.department Biomedical Sciences (Cellular & Molecular Biology) en_US
dc.date.graduated 2002-12 en_US
local.identifier.callnumber Q111 .H3 no. 3747 en_US
local.thesis.degreelevel MS en_US

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