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dc.contributor.author Taguchi, Satoru en_US
dc.contributor.author Laws, Edward A en_US
dc.date.accessioned 2008-11-03T06:45:01Z en_US
dc.date.available 2008-11-03T06:45:01Z en_US
dc.date.issued 2001-01 en_US
dc.identifier.citation Taguchi S, Laws EA. 2001. Dark protein synthesis: physiological response to nutrient limitation of a natural phytoplankton population. Pac Sci 55(1): 1-15. en_US
dc.identifier.issn 0030-8870 en_US
dc.identifier.uri http://hdl.handle.net/10125/3225 en_US
dc.description.abstract Dark 14CO2 incorporation into protein was determined from 24-hr incubations using size-fractionated natural phytoplankton populations from Kane'ohe Bay, Hawai'i, enriched with either ammonium or ammonium plus phosphorus. Response to ammonium addition was maximum at an ammonium concentration of 3-4 μM. Dark 14C02 assimilation was suppressed by addition of both ammonium and phosphorus, but percentage incorporation into protein was not significantly different from addition of ammonium alone. About 75 ± 1% of the 14C taken up by the cells was incorporated into either protein or low-molecular-weight intermediate compounds. Cells smaller than 10 μm showed little response to nutrient additions. However, cells in the 10- to 35-μm size fraction incorporated significantly more 14C into protein when nutrients were added. C:N ratios calculated from the percentage of 14C incorporated into protein were most variable temporally in the 10- to 35-μm size group and least variable in the picoplankton (0.2-2.0 μm). Nutrient limitation indices (NUs) calculated from the quotient of C:N ratios in control and nutrient-enriched cultures were not significantly different for the picoplankton and 2- to 10-μm size fraction. The NLI for the 10- to 35-μm size fraction was significantly lower and implied a modest degree of nutrient limitation. The results suggest that cells smaller than 10 μm are growing at close to nutrient-saturated rates much of the time in Kane'ohe Bay. However, larger cells appear to experience a significant degree of nutrient limitation at some times, particularly when chlorophyll a concentrations are less than about 1 mg m-3 • Dark protein synthesis appears to be a useful modification of previous methods based on the dark uptake of 14C02 for studying nutrient limitation. en_US
dc.language.iso en-US en_US
dc.publisher University of Hawai’i Press en_US
dc.title Dark Protein Synthesis: Physiological Response to Nutrient Limitation of a Natural Phytoplankton Population en_US
dc.type Article en_US
dc.type.dcmi Text en_US

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