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The Antimicrobial Effects Of Compound 48/80 On Tetrahymena Pyriformis In Vivo

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Title: The Antimicrobial Effects Of Compound 48/80 On Tetrahymena Pyriformis In Vivo
Authors: Lau, Clifford
Issue Date: 15 Jan 2014
Publisher: University of Hawaii at Manoa
Abstract: Compound 48/80, a well-known and potent mast cell degranulator, has recently been found to possess antimicrobial activity. This thesis describes studies on the mechanism of the antimicrobial action of compound 48/80 on the protozoan Tetrahymena pyriformis GL. T. pyriformis cell division was synchronized using the heat treatment method developed by Scherbaum and Zeuthen. When compound 48/80 was added at different times following the end of heat treatment (EHT), normally effective concentrations had no significant effect on cell division if added 60 minutes after EHT. A lapse of 60 minutes after EHT correlates to the G2 period in the cell cycle, indicating that compound 48/80 acts somewhere within the G2 phase. Synchronized cells treated with this compound at 25 ug/ml showed a 10 minute lag for the first division and a 30 minute lag for the second division when compared to cells without the drug. Incorporation studies of amino acids (14C), thymidine (methyl-14c), and uridine (2-14c) into proteins, DNA, and RNA showed that compound 48/80 affected the incorporation of all three precursors at concentrations that inhibited cell division. At 80 minutes after EHT, 25 ug/ml of compound 48/80 inhibited incorporation of precursors into proteins by 38%, DNA by 47%, and RNA by 32%. The surface-active property of compound 48/80 was compared to the surfactant Triton X-100, (Octyl Phenoxy Polyethoxyethanol) and distilled water using a volumetric method. When compared to the volume of distilled water, Triton X-100 (0.01%) reduced drop volume by 55% whereas compound 48/80 (0.01% and 0.05%) gave volumes of 99.5% and 99% respectively. These results indicate that compound 48/80's mode of action is not as a surfactant such as Triton X-100.
Pages/Duration: iv, 36 pages
URI/DOI: http://hdl.handle.net/10125/32031
Rights: All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Honors Projects for Biology



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