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The Enzyme-Linked Immunosorbent Assay (Elisa) for γ-C-Reactive Protein Detection

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Title: The Enzyme-Linked Immunosorbent Assay (Elisa) for γ-C-Reactive Protein Detection
Authors: Furusawa, Eiko
Advisor: Hokama, Yoshitsugi
Issue Date: 15 Jan 2014
Publisher: University of Hawaii at Manoa
Abstract: C-Reactive Protein is an acute phase protein found in elevated levels in the blood during virtually all diseases associated with active inflammation or tissue destruction, particularly in patients with rheumatoid diseases, acute infectious processes, postmyocardial infarction or surgery, advanced and widespread malignancy, and chronic infections. Tillet and Francis (1) first discovered the existence of this protein in the sera of patients suffering from pneumoccal pneumonia. The precipitation of this protein was achieved by the addition of C-polysaccharide (C-substance) from the pneumococcal bacterial cell wall to the acute-phase serum in the presence of calcium ions. C-polysaccharide is composed of bacterial mucopeptide constituents, ribitol phosphate, N-acetyl-galactosamine phosphate, glucose, N-acetyl diaminotrideoxyhexose, and choline phosphate (2-4). The name "C-Reactive Protein" is based in this reaction of CRP to C-polysaccharide. CRP was detected by MacLeod and Avery (5,6) by capillary precipitation and complement fixation techniques with rabbit anti-CRP antibody (CRPA) . McCarty (7) crystallized rabbit ex-reactive protein (CxRP) and showed that it is analogous to the human CRP which appears in sera during inflammation.
Pages/Duration: iii, 42 pages
Rights: All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Honors Projects for Biology

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