Reisolation of a 50,000 Dalton Anorexigenic Protein from Rat Urine

Abstract

A 50,000 dalton protein has been successfully reisolated from rat urine. This protein has the property of reducing food intake when injected into rats and is the only natural substance known to be effective for long (24 hour) time periods. Successful reisolation involved use of a Cibacron blue-Sepharose affinity chromatography, DEAE-Sephacel ion exchange chromatography, Sephacryl S-400 chromatography, and SDS disc preparatory electrophoresis. It is likely to be the same 50,000 dalton isolated previously and has been found to contain no detectable carbohydrate. The present purification scheme appears to be more powerful than previous methodologies and may be superior in isolating the 50,000 dalton anorexigen from samples which are very badly contaminated.