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Studies on the Pepsin Hydrolysis of Chicken and Pheasant IgG; the Effect of 1.5 M NaCl on Pepsin F(ab1)2 Fragments

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Title: Studies on the Pepsin Hydrolysis of Chicken and Pheasant IgG; the Effect of 1.5 M NaCl on Pepsin F(ab1)2 Fragments
Authors: Hashiro, Glenn
Issue Date: 15 Jan 2014
Publisher: University of Hawaii at Manoa
Abstract: Chicken IgG digested with 1-2% pepsin at pH 4.5 for five to 18 hours yielded materials shown by ultracentrifugal analysis to be 75, 5S and 3.55. The 3.55 material was isolated by gel filtration in Sephadex G-100 equilibrated with 0.15 M NaCl (low salt), but the 75 and 55 materials could not be separated from each other by this method. In Sephadex G-200 equilibrated with 1.5 M NaCl (high salt), the 75 (apparently undigested IgG) aggregated to 115 (as was shown by ultracentrifugal analysis) and was separable from the 5S material. Gel diffusion, immunoelectrophoresis and radioimmunoelectrophoresis showed the isolated 55 fraction to be F(ab')2 with undigested IgG contaminants. The yield of chicken F(ab')2 was small and attempts were made to find conditions for obtaining better yields.
Pages/Duration: 55 pages
URI/DOI: http://hdl.handle.net/10125/31927
Rights: All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Honors Projects for Biology



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