Please use this identifier to cite or link to this item: http://hdl.handle.net/10125/31908

The Purification and Molecular Weight of Bovine Enterokinase

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Title: The Purification and Molecular Weight of Bovine Enterokinase
Authors: Fong, Lucy
Issue Date: 15 Jan 2014
Publisher: University of Hawaii at Manoa
Abstract: A purification procedure has been developed for bovine enterokinase. Material extruded from bovine duodena was extracted with 1% Triton-X-100. The extract was purified on a diethyl amino ethyl cellulose column run in the presence of 0.2% Triton. The eluate was loaded on to a pancreatic trypsin inhibitor- Sepharose affinity column and eluted with 0.5M KSCN. The final step of purification is gel filtration on a Sephacryl S-300 column. Yield was about 50% with about 1920 fold purification.
Pages/Duration: iv, 29 pages
URI/DOI: http://hdl.handle.net/10125/31908
Rights: All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Honors Projects for Biology



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