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An Analysis of the Effects of Ca++ on Flagellar Waveform

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Title: An Analysis of the Effects of Ca++ on Flagellar Waveform
Authors: Chun, Jerold
Issue Date: 15 Jan 2014
Publisher: University of Hawaii at Manoa
Abstract: Flagellar axonemes can be exposed by demembranation following which waveforms can be examined by suspending these axonemes in an ATP containing buffer and observing their motility through clarkfield microscopy. Sperm demembranated in the presence of ca++ with the non-ionic detergent Triton X-100 exhibit symmetrical waveforms upon reactivation and it was this phenomenon that was examined. Four factors, which affected symmetrization were identified and qualitatively characterized. These were the variables of sperm density, [Ca++], [Triton X-100], and the incubation period with respect to temperature. A fifth factor was examined - pH - however it had no effect.
Pages/Duration: 33 pages
URI/DOI: http://hdl.handle.net/10125/31905
Rights: All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Honors Projects for Biology



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