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Antibacterial activity of Vibrio sp. strain OCN008, a bacterium isolated from the coral Porites compressa

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Title: Antibacterial activity of Vibrio sp. strain OCN008, a bacterium isolated from the coral Porites compressa
Authors: Ono, Yuho
Instructor: Callahan, Sean
Issue Date: 09 May 2011
Publisher: University of Hawaii at Manoa
Abstract: Corals of Hawaii are increasingly experiencing outbreaks of diseases. One such disease is Montipora White Syndrome (MWS), which is hypothesized to be caused by marine bacteria. This disease causes rapid tissue loss in Montipora capitata, one of the dominant scleractinian coral species of Kaneohe Bay, Oahu, Hawaii. Vibrio sp. strain OCN008 was isolated from Porites compressa, another common species of coral in Kaneohe Bay, and was shown to inhibit the growth of three putative pathogens isolated from the coral mucus of MWS-affected M. capitata. OCN008 exhibited inhibitory activity on agar plate tests, including soft-agar overlay assays, culture droptests, and supernatant droptests. Inhibitory activity was also observed through growth curves of these putative pathogens incubated in 50% conditioned liquid medium and 50% GASW medium. The antibacterial substance was stable up to 100°C and has a molecular weight less than 3 kD. Significant pH changes were not observed in the conditioned medium, eliminating the possibility of inhibition caused by secretion of organic acids or hydrogen peroxide. The spectrum of inhibitory activity of this substance was also tested against various Gram-positive and Gram-negative bacteria, and was seen to inhibit the growth of Bacillis megaterium, Bacillus subtilis, Micrococcus luteus, and Staphylococcus saprophyticus. Further investigation on the physical nature and solubility of the antibacterial substance is required for isolation and identification of the inhibitory substance.
Pages/Duration: 53 pages
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Appears in Collections:Honors Projects for Biology

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