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Amplicon Engineering for Improved Bacterial Detection Using Loop-Mediated Isothermal Amplification (LAMP)

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Title: Amplicon Engineering for Improved Bacterial Detection Using Loop-Mediated Isothermal Amplification (LAMP)
Authors: Miyamoto, Adam
Instructor: Jenkins, Daniel
Issue Date: 09 May 2010
Publisher: University of Hawaii at Manoa
Abstract: Ralstonia solanacearum is a bacterium which induces wilt in plants, and is best described as a species complex due to variability in host range, metabolism, and other biological characteristics (Kubota, Vine, Alvarez, Jenkins, 2008). R. solanacearum populations are found primarily in warm, humid areas of the tropics and sub-tropics having originated from various countries through this belt (“Ralstonia,” 2008). Although some strains posing the greatest risk to agriculture are contained to limited regions of the world, global trade in agricultural materials make R. solanacearum a threat to crop producing economies worldwide. In particular, R. solanacearum Race 3 Biovar 2 has been identified as a select agent in the Agricultural Bioterrorism Act of 2002 by the USDA (“Ralstonia,” 2008) due to its cold-tolerance which makes it a major risk to the potato industry in temperate regions such as the US and Canada.
Pages/Duration: 17 pages
Rights: All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Honors Projects for Bioengineering

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