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dc.contributor.author Chua-Chiaco, Barrie Wu en_US
dc.date.accessioned 2012-02-03T21:36:33Z en_US
dc.date.available 2012-02-03T21:36:33Z en_US
dc.date.issued 1998-08 en_US
dc.identifier Q111 .H3 no.3376 en_US
dc.identifier.citation Chua-Chiaco BW. 1998. Effect of bioaugmentation and diesel fuel type on soil bioremediation. Honolulu (HI): Water Resources Research Center, University of Hawaii at Manoa. WRRC unedited report, 1998-05. en_US
dc.identifier.uri http://hdl.handle.net/10125/21940 en_US
dc.description Thesis (M.S.)--University of Hawaii at Manoa, 1998. en_US
dc.description Includes bibliographical references (leaves 106-117). en_US
dc.description Available also on microfiche. en_US
dc.description.abstract The enhancement of bioremediation by bioaugmentation in soil contaminated with diesel fuel No. 2 and No. 6 (Bunker C) is uncertain. A clayey soil was contaminated with 6,000 mg of either diesel fuel per kg of soil and seeded (5 x 10-7 cells/g of soil) with a Hawaii soil bacterium (UH138) known to utilize several hydrocarbons. The soil was limed, fertilized, and incubated in jars at 30°C for several months. The concentrations of total petroleum hydrocarbons (TPH) and of polycyclic aromatic hydrocarbons (PAH) in soil were measured by gravimetry and immunoassay, respectively. Poisoned controls (0.6% HgCl2) were used to determine the extent of hydrocarbon degradation due to microbial activity. A rapid first order biodegradation of TPH (84% in 23 days) occurred in soil contaminated with diesel fuel No. 2, regardless of bacterial seeding. Biodegradation of PAH was linear and reached 84% by day 98 in both seeded and unseeded treatments. Bioaugmentation had no effect on bioremediation of diesel fuel No.2. The decrease in TPH and PAH was paralleled by an increase in populations of total bacteria, phenanthrene-degrading bacteria and microorganisms capable of utilizing hexadecane and diesel fuel No. 2 as well as by an enhancement in CO2 evolution by the soil. Indigenous Zygomycetes grew profusely in diesel fuel No. 2 contaminated soil. Cunninghamella echinulata var. echinulata was isolated from the soil and was shown to be able to utilize several hydrocarbons. Thus, Zygomycetes may have contributed to the rapid decrease in contaminant. In soil contaminated with diesel fuel No. 6, the measurements of TPH and PAH were more variable due to the uneven distribution of the product. No biodegradation of the contaminant occurred over a period of 138 days. The growth of Zygomycetes was scant. The counts of total bacteria remained unchanged after the addition of diesel fuel No. 6. However, counts of the indigenous phenanthrene-degrading bacteria increases dramatically ( 4 log units) during the first 54 days whereas the level of the seeded bacteria remained stable. The counts of mineral oil degraders decrease by 2 log units after day 2. Co2 evolution from the soil confirmed that diesel fuel No. 6 was not degraded by either the indigenous microflora or the seeded bacterium. Thus, diesel fuel No. 2 was highly degradable by the indigenous population, however, diesel fuel No. 6 was recalcitrant. en_US
dc.description.sponsorship Department of the Interior; U.S Geological Survey via Water Resources Research Center en_US
dc.format.extent xv + 117 pages en_US
dc.language.iso en-US en_US
dc.publisher Water Resources Research Center, University of Hawaii at Manoa en_US
dc.relation Theses for the degree of Master of Science (University of Hawaii at Manoa). Microbiology; no. 3376. en_US
dc.relation   en_US
dc.relation.ispartofseries WRRC Unedited Reports. en_US
dc.relation.ispartofseries 1998-05 en_US
dc.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner. en_US
dc.subject.lcsh Soil remediation—Technological innovations. en_US
dc.subject.lcsh Bioremediation--Technological innovations. en_US
dc.title Effect of bioaugmentation and diesel fuel type on soil bioremediation en_US
dc.type Thesis en_US
dc.type.dcmi Text en_US

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