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Assessment of four soil nematode communities in Hawaii by different methods

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Item Summary

Title: Assessment of four soil nematode communities in Hawaii by different methods
Authors: Quintero, Tonia G.
Issue Date: 2008
Abstract: Nematode communities are potentially excellent indicators of soil health. Assessing these nematodes communities using molecular and morphological techniques may seem straight forward. However, many challenges exist in implementing molecular techniques. First, molecular techniques employed to study nematode communities generally involve DNA extraction, PCR, cloning and molecular sequencing. Each of these steps can introduce bias into the analysis of a nematode community. Using morphology to identify and assess nematode soil health is cumbersome as well. Systematic knowledge of the nematode fauna is essential in order to assign nematodes to their appropriate classification. In new environments permanent slides and collaboration with laboratories that extensively work on nematode taxonomy for adequate identification of nematodes is essential. As a result, attempting to assess the health of soil should require multiple nematode faunal analyses over time which includes I) comparison of two methods, 2) Gap analyses and 3) extraction methods. It is believed that these experiments have provided pertinent information to help in the quest of measuring and monitoring soil health. However, there is not adequate information to confidently determine if the Manoa Falls Trail sites were "healthier" in comparison to the Whitmore site.
Description: Thesis (Ph.D.)--University of Hawaii at Manoa, 2008.
Nematode communities are potentially excellent indicators of soil health. Assessing these nematodes communities using molecular and morphological techniques may seem straight forward. However, many challenges exist in implementing molecular techniques. First, molecular techniques employed to study nematode communities generally involve DNA extraction, PCR, cloning and molecular sequencing. Each of these steps can introduce bias into the analysis of a nematode community. Using morphology to identify and assess nematode soil health is cumbersome as well. Systematic knowledge of the nematode fauna is essential in order to assign nematodes to their appropriate classification. In new environments permanent slides and collaboration with laboratories that extensively work on nematode taxonomy for adequate identification of nematodes is essential. As a result, attempting to assess the health of soil should require multiple nematode faunal analyses over time which includes (1) comparison of two methods, (2) Gap analyses and (3) extraction methods. It is believed that these experiments have provided pertinent information to help in the quest of measuring and monitoring soil health. However, there is not adequate information to confidently determine if the Manoa Falls Trail sites were "healthier" in comparison to the Whitmore site.
Includes bibliographical references (leaves 151-152).
Also available by subscription via World Wide Web
153 leaves, bound 29 cm
URI/DOI: http://hdl.handle.net/10125/20923
ISBN: 9780549596226
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Ph.D. - Tropical Plant Pathology



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