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dc.contributor.author Nasu, Masaki en_US
dc.date.accessioned 2011-07-22T00:06:10Z en_US
dc.date.available 2011-07-22T00:06:10Z en_US
dc.date.issued 2006 en_US
dc.identifier.uri http://hdl.handle.net/10125/20740 en_US
dc.description Thesis (M.S.)--University of Hawaii at Manoa, 2006. en_US
dc.description Includes bibliographical references (leaves 54-56). en_US
dc.description vii, 56 leaves, bound ill. 29 cm en_US
dc.description.abstract The lyretail swordtail fish is a dominant mutant trait of common swordtail (Xiphophorus helleri). Three main approaches were used to identify the DNA markers to distinguish homozygous dominant lyretail from heterozygous lyretail. 1) Microsatellite primers, 2) Random amplified polymorphic DNA (RAPD), and 3) Suppression subtractive hybridization (SSH) cDNA library. One of the microsatellite primers, KonT38, revealed 250-bp DNA fragment in both common and heterozygous lyretails, but not in homozygous lyretails. Therefore this DNA fragment may be related to the for a recessive allele for lyretail phenotype. More than 650 reactions of RAPD showed many polymorphic DNA fragments. However, specific banding patterns related to the lyretail genotypes, which could be used as DNA markers, did not appear. A number of differentially expressed genes in the lyretail and common fins have also been identified through SSH analyses. The differentially expressed cDNAs isolated from the lyretail library include four genes for cell proliferation, while the cDNAs identified from common fin include genes for regulation of cell proliferation and inhibition of gene expressions. en_US
dc.language.iso en-US en_US
dc.relation Theses for the degree of Master of Science (University of Hawaii at Manoa) no. 4132 en_US
dc.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner. en_US
dc.subject Swordtails (Fish) -- Genetics en_US
dc.title Identification of DNA markers for the homozygous lyretail in swordtail fish en_US
dc.type Thesis en_US
dc.type.dcmi Text en_US

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