ScholarSpace will be brought offline for upgrades on Wednesday December 9th at 11AM HST. Service will be disrupted for approximately 2 hours. Please direct any questions to

Item Description

Show full item record

Title: Development and testing of microsatellite markers for genetic mapping in papaya 
Author: Eustice, Moriah Rose
Date: 2006
Abstract: Microsatellites, or simple sequence repeats (SSRs), are tandem1y repeated mono-to hexanucleotide DNA motifs. Due to their co-dominant nature, ability to detect high levels of allelic diversity and ubiquitous genome distribution, they are widely used as genetic markers for diversity analysis and mapping projects. SSRs are also thought to serve a functional purpose in coding (genic) regions, where they have recently received considerable attention due to their role in several human diseases. Genomic research on papaya (Carica papaya L.) has yielded significant quantities of sequence data, which can be used to efficiently and economically develop microsatellites. The present project mined 2S.1 Mb of BES, 5.S Mb cDNA and 1.6 Mb random genomic sequences for SSRs. A total of939 SSR markers were identified with sufficient flanking sequences to design primers for PCR amplification. Of these, 831 produced acceptable amplicons (88.5%) with 444 displaying polymorphism (53.4%). Among seven parental varieties of five mapping populations tested, Kaek Dhom x 2H94 had the highest level of polymorphism with 210 markers followed by UH928 x SunUp with 194. AU9x SunUp with 189, UH91S x SunUp with 177 and Kapoho x SunUp, with 97, had the fewest. Dinucleotides were the most predominant motif class, accounting for 695 of the 939 mined SSRs (74.0%). The repeat motif AT/TA alone accounted for 481 SSRs (51.2%). Differences were observed between the genomic and genic fractions in levels of polymorphism, motif class predominance and motif length, indicating different selection pressures acting on SSRs between these two regions. The availability of the SSRs developed in this study will greatly assist in the genetic and physical mapping of the papaya genome as well as enhance the capacity for papaya improvement.
Description: Thesis (M.S.)--University of Hawaii at Manoa, 2006. Includes bibliographical references (leaves 53-67). x, 67 leaves, bound ill. 29 cm
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Keywords: Papaya -- Genome mapping

Item File(s)

Description Files Size Format View
Restricted for viewing only M.S.Q111.H3_4082_r.pdf 1.536Mb PDF View/Open
For UH users only M.S.Q111.H3_4082_uh.pdf 1.532Mb PDF View/Open

This item appears in the following Collection(s)


Advanced Search


My Account