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Investigating the cause for the difference in SPEB activity between two related Streptococcus pyogenes isolates

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Item Summary

Title: Investigating the cause for the difference in SPEB activity between two related Streptococcus pyogenes isolates
Authors: Garcia, Alan F.
Keywords: Streptococcus pyogenes -- Genetics
Issue Date: 2006
Abstract: Two emm 81 throat and blood streptococcus pyogenes isolates were obtained 13 days apart from an individual patient. They were genetically similar but showed multiple phenotypic differences, the most remarkable being the complete lack of streptococcal pyrogenic exotoxin S (speB) functional activity in the blood but not the throat isolate. PFGE analysis using 5 different restriction enzymes and sequencing of selected genes confirmed their genetic similarity. Immunoblots of culture supernatants established that the speB was secreted only by the throat isolate. Immunoblots of cell lysates showed that both produced the SpeB precursor but only the throat isolate processed the mature form. Real time RT-PCR established differences in speB transcription. Microarray analysis comparing the two isolates revealed many transcriptional differences suggesting that a regulatory gene, perhaps due to an epigenetic change, is responsible for the observed phenotypic differences of these two isolates.
Description: Thesis (M.S.)--University of Hawaii at Manoa, 2006.
Includes bibliographical references (leaves 88-92).
xiv, 92 leaves, bound ill. (some col.) 29 cm
URI/DOI: http://hdl.handle.net/10125/20441
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:M.S. - Biomedical Sciences (Tropical Medicine)



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