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Title: The lipids of Rhodomicrobium vannielii 
Author: Park, Chong Eel
Date: 1966
Publisher: [Honolulu]
Abstract: Cells of Rhodomicrobium vannielii grown at 29° in a lactate-containing medium were extracted at room temperature with organic solvents. This extractable fraction contains the bulk of simple lipids (1.8% of cell dry weight) and complex lipids (phospholipids, 4.2%, and sulfolipid, 0.01%) coenzyme Q (0.09%) and pigments (carotenoids, 1.2%; and bacteriochlorophyll, 1.9%). The cell-residue contains the bound lipids (2.7%) which are liberated by treatment with alkali. The residue also contains poly-β-hydroxybutyric acid (0.2%), which was extracted with boiling CHCl3. CoQ was. determined to be Q9. Bacteriochlorophyll was found to be type a. Vaccenic acid (C18:1 ) is the major fatty acid component in both simple lipid and phospholipid fraction (ca. 90% in each fraction). The bound lipids contain many fatty acid components, including cyclopropane-, branched-, and alpha(α) and beta-(β)-hydroxy fatty acids. Seven carotenoids were found. Rhodopin (61.3% of total carotenoids by weight) and lycopene (20.5%) are the major constituents; the presence of β-carotene (3.4%) makes R. vannielii unique among the photosynthetic bacteria. The rest of the minor carotenoids are spirilloxanthin (11.2%), anhydro-rhodovibrin (1.8%), rhodovibrin (0.5%), and monodemethylated spirilloxanthin (0.5%). R. vannielii synthesizes mainly the normal spirilloxanthin series and is characterized by a high content of hydroxylated carotenoids. Crude phospholipids were separated from the bacteriochlorophyll by acetone treatment after fractionation on silicic acid columns. They were refractionated by TLC on silica gel-H and 8 components were separated; 7 of these contained phosphorus. The nature and amounts of these components were determined. They are phosphatidic acid (1.83% of total phospholipids by weight), bis-phosphatidic acid (6.75%), phosphatidyl ethanolamine (4.5%), an ornithine ester of phosphatidyl glycerol (46.5%), phosphatidyl glycerol (9.7%), an ornithine amide of an undetermined fatty acid (0.95%), phosphatidyl choline (26.5%), and the ornithine ester of lysophosphatidyl glycerol (3.2%).
Description: Typescript. Thesis (Ph. D.)--University of Hawaii, 1966. Bibliography: leaves [85]-96. 96 l illus., tables
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Keywords: Lipids, Rhodomicrobium vannielii

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