ScholarSpace will be brought offline for upgrades on Wednesday December 9th at 11AM HST. Service will be disrupted for approximately 2 hours. Please direct any questions to

Item Description

Show full item record

Title: Isolation and characterization of messenger ribonucleic acid for hemoglobin 
Author: Laycock, David Gerald
Date: 1969
Publisher: [Honolulu]
Abstract: An 8s ribonucleic acid (RNA) species, isolated from rabbit reticulocytes, has been purified by salt fractionation and analyzed for characteristics of an information-carrying, messenger RNA. This RNA in reticulocytes is restricted to polyribosome complexes which are active in hemoglobin synthesis and has a base composition similar to that expected of a messenger RNA for rabbit globin. This RNA species has been assayed for the ability to support and direct protein synthesis in bacterial extracts. The 8s RNA from reticulocyte polyribosomes is translated when only N-acetyl-valyl- tRNA is present as an initiation residue. Both rabbit globin polypeptide chains have N-terminal valine residues. The protein produced in bacterial extracts directed by this RNA is identical to rabbit globin by chromatography on diethylaminoethyl-cellulose, carboxymethyl-Sephadex C50, paper electrophoresis and gel-filtration on Sephadex G75. The protein product combines with added hemin and the resultant complex is indistinguishable from native rabbit hemoglobin by the above criteria. The profile of tryptic peptides from chromatography on Dowex 50x2 indicates the product is N-acetyl globin. The implications of these results concerning the universality of the genetic code and of protein biosynthetic mechanisms at a macromolecular level are discussed.
Description: Typescript. Thesis (Ph. D.)--University of Hawaii, 1969. Bibliography: leaves 109-114. viii, 114 l illus
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Keywords: RNA, Hemoglobin

Item File(s)

Description Files Size Format View
Restricted for viewing only uhm_phd_6916657_r.pdf 3.614Mb PDF View/Open
For UH users only uhm_phd_6916657_uh.pdf 3.580Mb PDF View/Open

This item appears in the following Collection(s)


Advanced Search


My Account