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|Title:||The effect of steroids on RNA synthesis in various tissues of the rat|
|Authors:||Philleo, William Wallace|
|Abstract:||Single doses of aldosterone were administered to normal rats, and the effect of RNA synthesis was studied. The maximal stimulation of the incorporation of l4c-ATP into RNA by nuclei isolated from rat kidneys was observed 30 minutes after an intravenous injection of aldosterone. Thereafter, the RNA synthesis decreased to a level less than that of the control, reaching a minimum at 2.5 hours. The return to control level was followed by further oscillations in RNA synthesis. Likewise, in isolated brain nuclei, an oscillation in RNA synthesis was observed following an intravenous injection of aldosterone. The effect of single injections of aldosterone, cortisol, deoxycorticosterone acetate, testosterone or progesterone was studied on RNA synthesis by isolated rat spleen nuclei. It was found that the l4C-ATP incorporation was initially inhibited following the injection of aldosterone, cortisol or deoxycorticosterone acetate. The initial response to an injection of testosterone or progesterone was a stimulation in the spleen RNA synthesis. The response to the administration of aldosterone was similar to that of cortisol. Subsequent to the initial inhibition, a stimulation followed by a return to the level of the control, was observed and secondary inhibitions were found. RNA synthesis in the thymus was initially inhibited following the administration of aldosterone. RNA synthesis was studied in the nuclei isolated from the kidney and spleen of adrenalectomized or hypophysectomized rats injected with aldosterone. Oscillations were observed in the RNA synthesis of both tissues of the endocrinectomized rats. It was concluded that the oscillations in RNA synthesis were not a function of some form of homeostatic control involving the pituitary and adrenal glands. Aldactone (SC 9420) was found to modify the RNA synthesis of nuclei isolated from the kidney, spleen and thymus of intact, adrenalectomized or hypophysectomized rats. Again, oscillations in RNA synthesis were observed, however there was the suggestion that the adrenals or the pituitary might be involved in the response as observed in the kidney and the thymus. Experiments were carried out in which both aldosterone and Aldactone were injected, and their action on RNA synthesis was observed in the kidney, spleen and thymus. The results indicated that these two steroids alter RNA synthesis independently from each other. Analysis of the RNA synthesized in vitro by the isolated nuclei of the kidney and spleen indicated that only a single species of RNA was made. This 5 s RNA was identical to that species of RNA present in nuclei, prior to incubation for the incorporation of l4C-ATP.|
Thesis (Ph. D.)--University of Hawaii, 1969.
Bibliography: leaves 136-144.
xii, 144 l
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|Appears in Collections:||Ph.D. - Biomedical Sciences (Biochemistry)|
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