Please use this identifier to cite or link to this item:

The behavior of horse liver alcohol dehydrogenase in guanidine hydrochloride solutions

File Description SizeFormat 
uhm_phd_6916653_r.pdfVersion for non-UH users. Copying/Printing is not permitted2.19 MBAdobe PDFView/Open
uhm_phd_6916653_uh.pdfVersion for UH users2.15 MBAdobe PDFView/Open

Item Summary

Title: The behavior of horse liver alcohol dehydrogenase in guanidine hydrochloride solutions
Authors: Green, Robert William
Keywords: Dehydrogenases
Issue Date: 1969
Publisher: [Honolulu]
Abstract: The Z average molecular weight of horse liver alcohol dehydrogenase (LADH) in dilute neutral buffer was found to be 78,200 by sedimentation equilibrium. One molar guanidine hydrochloride (GuHCl) reversibly inhibited LADH activity. The inhibition was competitive with limiting nicotinamide adenine dinucleotide and mixed with limiting ethanol. When EDTA was added to LADH in 1 M GuHCl, zinc was removed followed by aggregation. Sedimentation velocity experiments indicated that no subunit was formed under these conditions. Higher concentrations of GuHCl resulted in dissociation of LADH. In 3 M GuHCl, the sedimentation equilibrium data indicated the presence of a reversible association-dissociation system involving subunit, dimer, and trimer. EDTA removed zinc but had no effect on the molecular weight of LADH in 3 M GuHCl. Reduction and alkylation of LADH in 3 M GuHCl resulted in almost complete dissociation into two subunits. Apparently, sulfhydryl residues are involved in subunit association, while zinc plays little or no role. The Z average molecular weight of LADH in 5 M GuHCl containing mercaptoethanol was found to be 45,700, a value higher than expected. The discrepancy may be attributed to incomplete dissociation, a small error in apparent partial specific volume, or solvent binding. Measurements of molecular weight, sedimentation coefficient, and intrinsic viscosity of LADH in 5 M GuHCl indicate that the structure of LADH is composed of two subunits.
Description: Typescript.
Thesis (Ph. D.)--University of Hawaii, 1969.
Bibliography: leaves 110-113.
xi, 113 l tables
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:Ph.D. - Biomedical Sciences (Biochemistry)

Items in ScholarSpace are protected by copyright, with all rights reserved, unless otherwise indicated.