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Title: Temporal Variation In An Initial Marine Biofilm Community And Its Effect On Larval Settlement Of The Tubeworm Hydroides Elegans
Authors: Shikuma, Nicholas J.
Issue Date: May-2005
Abstract: Planktonic larvae of many invertebrates settle preferentially on surfaces covered by bacterial biofilms. The polychaete tubeworm Hydroides elegans is induced to settle by biofilms and is the primary colonizer of newly submerged surfaces in the succession of macrofouling invertebrates in Pearl Harbor, Hawai'i. This study examines culture-independent community composition, as well as densities of bacteria, and how these aspects of marine biofilms affect settlement preferences of H. elegans. Settlement assays of H. elegans were conducted on naturally formed biofilms of increasing age from Pearl Harbor, Hawai'i. Denaturing Gradient Gel Electrophoresis (DGGE) and epifluoresence microscopy were used to identify community composition and densities of bacterial biofilms. This study showed that increased densities of bacteria rather than dominant species composition are likely responsible for the primary colonization of submerged surfaces by H. elegans in Pearl Harbor.
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Appears in Collections:M.S. - Zoology

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