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Aluminum tolerance in Medicago truncatula Gaertn

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Item Summary

Title: Aluminum tolerance in Medicago truncatula Gaertn
Authors: Srimake, Yawadee
Keywords: aluminum toxicity
Medicago truncatula
acid soil
root growth
Issue Date: May 2012
Publisher: [Honolulu] : [University of Hawaii at Manoa], [May 2012]
Abstract: Aluminum (Al) toxicity in acid soils is one of the major problems in agricultural production worldwide. In this study, we investigated Al tolerance in a legume crop, Medicago truncatula. Ninety-one ecotypes and 62 ethylmethylsulfonate (EMS)-generated mutants were screened for their Al-sensitivity using three different methods: 1) Al-containing agarose media; 2) hematoxylin staining; and 3) Al-toxic soil. Root lengths and Relative Root Growth (RRG, i.e. ratio of root elongation of seedlings grown with Al at a particular level to that of seedlings grown without Al) were measured. We found significant differences in response to Al-toxicity among the ecotypes and EMS mutant lines, and the results correlated positively between each method. We identified 3 Al-tolerant, 3 sensitive ecotypes, and 1 sensitive mutant. The Al-tolerant and sensitive lines were cross-pollinated to generate segregating populations. The F2 population of PI 577633 (Al-tolerant ecotype) and S69 (sensitive mutant) were screened using the Al-containing agarose assay. Genomic DNA of extremely tolerant and sensitive seedlings was extracted for Restriction site-associated DNA (RAD) analysis. We identified 11 candidate alleles that might be associated with Al tolerance. This information could be useful in breeding or selecting for improved Al tolerance in the model legume M. truncatula, as well as other crop legume species.
Description: Ph.D. University of Hawaii at Manoa 2012.
Includes bibliographical references.
Appears in Collections:Ph.D. - Tropical Plant and Soil Sciences

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