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An optically addressed thermoresponsive microfluidic system for cell culturing and harvesting
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|Title:||An optically addressed thermoresponsive microfluidic system for cell culturing and harvesting|
|Authors:||Namekar, Swapnil Arun|
|Keywords:||thermoresponsive microfluidic system|
|Issue Date:||Aug 2012|
|Publisher:||[Honolulu] : [University of Hawaii at Manoa], [August 2012]|
|Abstract:||The present work demonstrates a "smart" culture platform that can be used for cell culturing and harvesting specific cells, such as stem cells. It is desirable to culture homogeneous specific cell populations for various biomedical applications such as fundamental cellular studies and studies of tissues. Traditional cell culturing techniques lack control over the spatial location of individual cells in a culture, or the types of cells that are seeded. These limitations hinder studies on cell-cell interactions and the creation of completely homogeneous populations of cells. In order to overcome these limitations, a smart culture dish was developed that enables control of specific individual cells in a cell culture by using optical illumination. In addition, a method for seeding specific individual cells in a three-dimensional culture using optical illumination was demonstrated. Both of these techniques can individually be used for achieving control over specific individual cells and their spatial location in a culture. This further enables the culturing and harvesting of specific individual cell types, and the creation of homogeneous specific cell populations.|
|Description:||M.S. University of Hawaii at Manoa 2012.|
Includes bibliographical references.
|Appears in Collections:||M.S. - Electrical Engineering|
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