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Study of mature dengue virus-like particles as new dengue vaccine candidate

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Item Summary

Title: Study of mature dengue virus-like particles as new dengue vaccine candidate
Authors: Lloyd, Yukie Michelle
Keywords: monoclonal
Issue Date: Aug 2014
Publisher: [Honolulu] : [University of Hawaii at Manoa], [August 2014]
Abstract: Our study of different human monoclonal antibodies showed that compared with group reactive anti-fusion loop monoclonal antibodies, type specific antidomain III monoclonal antibodies have higher epitope accessibility and binding avidity to mature virus-like particles (VLPs), which presumably representing mature dengue virus (DENV) virions, the major infectious component in mixed DENV particles in culture. These findings not only suggest that type specific antibodies such as anti-domain III antibodies, though present as a small proportion of anti-envelope antibodies in polyclonal human serum, may contribute greatly to neutralizing activity, but also highlight the importance of inducing this type specific anti-domain III antibodies by using mature particles for vaccine strategy. Using DNA vaccines to test our hypothesis, we showed that DNA vaccine expressing mature VLPs could induce similar anti-DENV antibodies, neutralizing antibodies, less anti-fusion loop antibodies, no or little anti-precursor membrane antibodies compared with DNA vaccine expressing mixed VLPs. Future studies are needed to verify that mature DENV particles are better vaccine candidates than mixed DENV particles.
Description: M.S. University of Hawaii at Manoa 2014.
Includes bibliographical references.
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:M.S. - Biomedical Sciences (Tropical Medicine)

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