http://hdl.handle.net/10125/868 Wed, 19 Jun 2013 22:58:36 GMT 2013-06-19T22:58:36Z http://hdl.handle.net/10125/9405 Thesis (Ph. D.)--University of Hawaii at Manoa, 1996.; Includes bibliographical references (leaves 185-196).; Microfiche.; xii, 196 leaves, bound ill. (some col.) 29 cm Mon, 01 Jan 1996 00:00:00 GMT http://hdl.handle.net/10125/9405 1996-01-01T00:00:00Z Yamashita, Roxanne http://hdl.handle.net/10125/9404 Thesis (Ph. D.)--University of Hawaii at Manoa, 1996.; Includes bibliographical references (leaves 159-175).; Microfiche.; xv, 175 leaves, bound ill. 29 cm Mon, 01 Jan 1996 00:00:00 GMT http://hdl.handle.net/10125/9404 1996-01-01T00:00:00Z Anderson, John Bruce http://hdl.handle.net/10125/9403 Thesis (Ph. D.)--University of Hawaii at Manoa, 1995.; Includes bibliographical references (leaves 171-187).; Microfiche.; xix, 187 leaves, bound ill. 29 cm Sun, 01 Jan 1995 00:00:00 GMT http://hdl.handle.net/10125/9403 1995-01-01T00:00:00Z Sujinda Thanaphum http://hdl.handle.net/10125/9402 Thesis (Ph. D.)--University of Hawaii at Manoa, 1994.; Includes bibliographical references (leaves 166-191).; Microfiche.; xiii, 191 leaves, bound ill. 29 cm Sat, 01 Jan 1994 00:00:00 GMT http://hdl.handle.net/10125/9402 1994-01-01T00:00:00Z Feldman, Robert A http://hdl.handle.net/10125/9401 Thesis (Ph. D.)--University of Hawaii at Manoa, 1994.; Includes bibliographical references (leaves 160-175); Microfiche.; 175 leaves, bound ill. 29 cm Sat, 01 Jan 1994 00:00:00 GMT http://hdl.handle.net/10125/9401 1994-01-01T00:00:00Z Wisotzkey, Robert Grier http://hdl.handle.net/10125/9400 Thesis (Ph. D.)--University of Hawaii at Manoa, 1993.; Includes bibliographical references (leaves 177-205); Microfiche.; xiii, 205 leaves, bound ill. 29 cm Fri, 01 Jan 1993 00:00:00 GMT http://hdl.handle.net/10125/9400 1993-01-01T00:00:00Z He, Mei http://hdl.handle.net/10125/9399 Thesis (Ph. D.)--University of Hawaii at Manoa, 1991.; Includes bibliographical references (leaves 192-206); Microfiche.; xi, 206 leaves, bound ill.,maps 29 cm Tue, 01 Jan 1991 00:00:00 GMT http://hdl.handle.net/10125/9399 1991-01-01T00:00:00Z Hall, Jana http://hdl.handle.net/10125/9398 Thesis (Ph. D.)--University of Hawaii at Manoa, 1990.; Includes bibliographical references (leaves 124-138); Microfiche.; xiii, 138 leaves, bound ill. 29 cm Mon, 01 Jan 1990 00:00:00 GMT http://hdl.handle.net/10125/9398 1990-01-01T00:00:00Z Titus, Elizabeth Anne Brumbaugh http://hdl.handle.net/10125/9397 Typescript.; Thesis (Ph. D.)--University of Hawaii at Manoa, 1990.; Includes bibliographical references (leaves 100-109); Microfiche.; viii, 109 leaves, bound ill. 29 cm Mon, 01 Jan 1990 00:00:00 GMT http://hdl.handle.net/10125/9397 1990-01-01T00:00:00Z Brezinsky, Laura http://hdl.handle.net/10125/9396 Typescript.; Thesis (Ph. D.)--University of Hawaii at Manoa, 1989.; Includes bibliographical references (leaves 165-174); Microfiche.; x, 174 leaves, bound ill. 29 cm Sun, 01 Jan 1989 00:00:00 GMT http://hdl.handle.net/10125/9396 1989-01-01T00:00:00Z Koo, Kenneth http://hdl.handle.net/10125/9395 Typescript.; Thesis (Ph. D.)--University of Hawaii at Manoa, 1984.; Bibliography: leaves 103-110.; Microfiche.; x, 110 leaves, bound ill., map 29 cm Sun, 01 Jan 1984 00:00:00 GMT http://hdl.handle.net/10125/9395 1984-01-01T00:00:00Z Yamada, Randolph http://hdl.handle.net/10125/9394 Typescript.; Thesis (Ph. D.)--University of Hawaii at Manoa, 1983.; Bibliography: leaves 283-290.; Microfiche.; xix, 290 leaves, bound ill. 29 cm Sat, 01 Jan 1983 00:00:00 GMT http://hdl.handle.net/10125/9394 1983-01-01T00:00:00Z Kitaji, Gail http://hdl.handle.net/10125/988 The objective of this dissertation is the isolation of DNA sequences that are differentially represented in males and females of the Oriental fruit fly Bactrocera dorsalis, specifically by initiating a molecular characterization of Y chromosome sequences in this species. Cytological observations have established the presence of a diminutive Y chromosome in B. dorsalis males. To isolate DNA sequences from the Y chromosome, a special method of genomic DNA isolation known as Representational Difference Analysis (RDA) was utilized to obtain DNA sequences unique to the B. dorsalis male genome. Genomic DNA from B. dorsalis males served as the "tester" DNA and female genomic DNA as the "driver" DNA. Six distinct RDA products were obtained following two complete rounds of DNA hybridization and difference enrichment via the Polymerase Chain Reaction (PCR). One ofthese products (RDA product 1) was used to isolate a genomic DNA clone (3.1a) from a B. dorsalis male genomic DNA minilibrary. This sequence shows similarity to the reverse transcriptase of R1 retrotransposable elements. The presence of R1 elements in the Tephritid insects has heretofore been undescribed, although these elements have been previously described in the genomes of other Dipteran species. Oligonucleotide primers for PCR were designed for the 3.1a clone. These primers consistently produce different amplification patterns in PCRs ofgenomic DNA from B. dorsalis males vs. females. Amplification using male genomic DNA produces 325 bp and 2.6 kb products while only a 2.6 kb product is obtained from female DNA. The amplification products obtained with these primers are also produced in PCRs of genomic DNA from B. dorsalis embryos and third instar larvae, suggesting the ability of this method to infer sex at pre-adult stages ofthe B. dorsalis life cycle. Similar amplification products have also been obtained in other Bactrocera species. Both the 325 bp male PCR product and the 2.6 kb products have regions of sequence similarity to R1 elements. The 2.6 kb product contains a putative 1.7 kb open reading frame (ORF) encoding 583 amino acids. Three amino acid motifs found in Drosophila R1 element reverse transcriptases are present in comparable locations within the hypothetical ORF product. Both of these sequences are also repetitively represented in the B. dorsalis male and female genomes. However, the 325 bp male product produces some bands that are male specific when used as a probe for Southern blots of B. dorsalis male and female genomic DNA. The amplification pattern produced by the 3.1a primers is consistent with what would be expected if the 2.6 kb and 325 bp PCR products originated from the B. dorsalis X and Y chromosomes, respectively. Thus, the cloned male-specific sequence recovered here is potentially useful both as a gateway into the relatively uncharacterized B. dorsalis Y chromosome and as a tool for the characterization of other aspects of the B. dorsalis genome. Sun, 01 Dec 2002 00:00:00 GMT http://hdl.handle.net/10125/988 2002-12-01T00:00:00Z Lai, Janice Su Yin