http://hdl.handle.net/10125/860 Sat, 18 May 2013 21:55:55 GMT 2013-05-18T21:55:55Z http://hdl.handle.net/10125/20433 Thesis (Ph. D.)--University of Hawaii at Manoa, 2008.; Includes bibliographical references (leaves 25-28).; Also available by subscription via World Wide Web; ix, 28 leaves, bound 29 cm Tue, 01 Jan 2008 00:00:00 GMT http://hdl.handle.net/10125/20433 2008-01-01T00:00:00Z McMurtray, Aaron http://hdl.handle.net/10125/20432 Thesis (M.S.)--University of Hawaii at Manoa, 2007.; Includes bibliographical references (leaves 18-20).; vii, 20 leaves, bound col. ill. 29 cm Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10125/20432 2007-01-01T00:00:00Z Niide, Tiffany K http://hdl.handle.net/10125/20431 Thesis (M.S.)--University of Hawaii at Manoa, 2007.; Includes bibliographical references.; viii, 12 leaves, bound ill. 29 cm Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10125/20431 2007-01-01T00:00:00Z Fu, Jennifer http://hdl.handle.net/10125/20430 Thesis (M.S.)--University of Hawaii at Manoa, 2007.; Includes bibliographical references (leaves 13-17).; vii, 17 leaves, bound ill. 29 cm Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10125/20430 2007-01-01T00:00:00Z McMurtray, Aaron http://hdl.handle.net/10125/20429 Thesis (M.S.)--University of Hawaii at Manoa, 2005.; Includes bibliographical references (leaves 32-35).; vii, 35 leaves, bound 29 cm Sat, 01 Jan 2005 00:00:00 GMT http://hdl.handle.net/10125/20429 2005-01-01T00:00:00Z Withy, Kelley http://hdl.handle.net/10125/20428 Thesis (M.S.)--University of Hawaii at Manoa, 2005.; Includes bibliographical references (leaves 25-29).; viii, 29 leaves, bound col. ill. 29 cm Sat, 01 Jan 2005 00:00:00 GMT http://hdl.handle.net/10125/20428 2005-01-01T00:00:00Z Okihiro, May http://hdl.handle.net/10125/20427 Thesis (M.S.)--University of Hawaii at Manoa, 2005.; Includes bibliographical references (leaves 29-31).; x, 31 leaves, bound 29 cm Sat, 01 Jan 2005 00:00:00 GMT http://hdl.handle.net/10125/20427 2005-01-01T00:00:00Z Kretzer, Kikikipa http://hdl.handle.net/10125/20426 Thesis (M.S.)--University of Hawaii at Manoa, 2005.; Includes bibliographical references.; vii, 23 leaves, bound ill. 29 cm Sat, 01 Jan 2005 00:00:00 GMT http://hdl.handle.net/10125/20426 2005-01-01T00:00:00Z Kim, David Seil http://hdl.handle.net/10125/20425 Thesis (M.S.)--University of Hawaii at Manoa, 2005.; Includes bibliographical references (leaves 34-36).; viii, 36 leaves, bound col. ill. 29 cm Sat, 01 Jan 2005 00:00:00 GMT http://hdl.handle.net/10125/20425 2005-01-01T00:00:00Z Day, Larry John http://hdl.handle.net/10125/20424 Thesis (M.S.)--University of Hawaii at Manoa, 2005.; Includes bibliographical references (leaves 37-41).; viii, 41 leaves, bound cil. ill. 29 cm Sat, 01 Jan 2005 00:00:00 GMT http://hdl.handle.net/10125/20424 2005-01-01T00:00:00Z Chapital, Alyssa Dianne http://hdl.handle.net/10125/20423 Thesis (M.S.)--University of Hawaii at Manoa, 2007.; Includes bibliographical references (leaves 14-16).; vi, 16 leaves, bound 29 cm Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10125/20423 2007-01-01T00:00:00Z Weiss, Lois http://hdl.handle.net/10125/12107 Mode of access: World Wide Web.; Thesis (Ph. D.)--University of Hawaii at Manoa, 2005.; Includes bibliographical references (leaves 158-166).; Electronic reproduction.; Also available by subscription via World Wide Web; xiii, 166 leaves, bound col. ill. 29 cm. + Sat, 01 Jan 2005 00:00:00 GMT http://hdl.handle.net/10125/12107 2005-01-01T00:00:00Z Killebrew, Deirdre Anne http://hdl.handle.net/10125/10398 Mon, 01 Aug 2005 00:00:00 GMT http://hdl.handle.net/10125/10398 2005-08-01T00:00:00Z Hassanin, Hanan http://hdl.handle.net/10125/9359 Thesis (Ph. D.)--University of Hawaii at Manoa, 1993.; Microfiche.; x, 140 leaves, bound ill. 29 cm Fri, 01 Jan 1993 00:00:00 GMT http://hdl.handle.net/10125/9359 1993-01-01T00:00:00Z Han, Hyo-Young http://hdl.handle.net/10125/9358 Thesis (Ph. D.)--University of Hawaii at Manoa, 1993.; Microfiche.; vii 70 leaves, bound ill. 29 cm Fri, 01 Jan 1993 00:00:00 GMT http://hdl.handle.net/10125/9358 1993-01-01T00:00:00Z Fleig, Andrea http://hdl.handle.net/10125/6983 Many species are known to be part of groupings of extremely closely related species known as species complexes. The Bactrocera dorsalis species complex contains at least 52 closely related species, many of which are major economic pests. In addition, many of them cannot be accurately identified using traditional approaches and very little is known about their systematic relationships. DNA markers may provide useful characters for both purposes. To develop a set of DNA markers, primers for per were designed from conserved coding regions of an actin gene (BdA1) previously characterized from B. dorsalis. These were used to amplify the intron region of this gene from known Bactrocera specimens including three species found within the complex and two from outside the complex. The amplification products obtained were cloned into plasmid vectors and sequenced. Analysis of these intron sequences revealed polymorphisms within and between species. From these, ten alleles were identified. Oligonucleotides corresponding to these alleles were synthesized and used to construct DNA arrays. The use of these arrays for genotyping and species identification was validated using a series of known specimens. This approach may also be useful for other applications including identification of unknown individuals, resolution of systematic relationships, monitoring routes of dispersal and assessing the origins of new infestations of these pests. The ability to rapidly and accurately identify species causing infestations may be a significant factor in reducing the damage and economic impact of such pests. xii, 74 leaves Thu, 01 May 2003 00:00:00 GMT http://hdl.handle.net/10125/6983 2003-05-01T00:00:00Z Naeole, Chrystie KM