http://hdl.handle.net/10125/589 Wed, 19 Jun 2013 13:49:04 GMT 2013-06-19T13:49:04Z http://hdl.handle.net/10125/20397 Thesis (M.S.)--University of Hawaii at Manoa, 2007.; Includes bibliographical references (leaves 61-66).; vii, 66 leaves, bound ill. 29 cm Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10125/20397 2007-01-01T00:00:00Z Buck, Kevin http://hdl.handle.net/10125/20396 Thesis (M.S.)--University of Hawaii at Manoa, 2007.; Includes bibliographical references (leaves 45-55).; i, 92 leaves, bound ill. 29 cm Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10125/20396 2007-01-01T00:00:00Z Bobbili, Naveen K http://hdl.handle.net/10125/20395 Thesis (M.S.)--University of Hawaii at Manoa, 2006.; Includes bibliographical references (leaves 65-78).; xi, 91 leaves, bound ill. ( some col.) 29 cm Sun, 01 Jan 2006 00:00:00 GMT http://hdl.handle.net/10125/20395 2006-01-01T00:00:00Z Putluru, Ravi K http://hdl.handle.net/10125/20394 Thesis (M.S.)--University of Hawaii at Manoa, 2006.; Includes bibliographical references (leaves 62-77).; ix, 77 leaves, bound ill. (some col.), maps (some col.) 29 cm Sun, 01 Jan 2006 00:00:00 GMT http://hdl.handle.net/10125/20394 2006-01-01T00:00:00Z Maresca, Barbara Tang http://hdl.handle.net/10125/20393 Thesis (M.S.)--University of Hawaii at Manoa, 2008.; Includes bibliographical references (leaves 79-82).; xii, 82 leaves, bound ill. 29 cm Tue, 01 Jan 2008 00:00:00 GMT http://hdl.handle.net/10125/20393 2008-01-01T00:00:00Z Witt, Elisha M http://hdl.handle.net/10125/20392 Thesis (M.S.)--University of Hawaii at Manoa, 2007.; Includes bibliographical references (leaves 68-69).; ii, 80 leaves, bound ill. 29 cm Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10125/20392 2007-01-01T00:00:00Z VanderLugt, Kyle R http://hdl.handle.net/10125/20391 Thesis (M.S.)--University of Hawaii at Manoa, 2008.; Includes bibliographical references (leaves 118-159).; xi, 159 leaves, bound 29 cm Tue, 01 Jan 2008 00:00:00 GMT http://hdl.handle.net/10125/20391 2008-01-01T00:00:00Z Johnson, Drew Leland http://hdl.handle.net/10125/10389 Mon, 01 Aug 2005 00:00:00 GMT http://hdl.handle.net/10125/10389 2005-08-01T00:00:00Z Humphreys, Vanessa http://hdl.handle.net/10125/10388 Wed, 01 Dec 2004 00:00:00 GMT http://hdl.handle.net/10125/10388 2004-12-01T00:00:00Z Watson, Michelle L. http://hdl.handle.net/10125/10387 Sat, 01 May 2004 00:00:00 GMT http://hdl.handle.net/10125/10387 2004-05-01T00:00:00Z Morishige, Carey L. http://hdl.handle.net/10125/10386 Sat, 01 May 2004 00:00:00 GMT http://hdl.handle.net/10125/10386 2004-05-01T00:00:00Z Gusman, Veronica M. http://hdl.handle.net/10125/10385 Sun, 01 May 2005 00:00:00 GMT http://hdl.handle.net/10125/10385 2005-05-01T00:00:00Z Leedom, Thomas A. http://hdl.handle.net/10125/10384 Sun, 01 May 2005 00:00:00 GMT http://hdl.handle.net/10125/10384 2005-05-01T00:00:00Z Fox, Bradley K. http://hdl.handle.net/10125/10383 Wed, 01 Dec 2004 00:00:00 GMT http://hdl.handle.net/10125/10383 2004-12-01T00:00:00Z Keala, Noniponimo'i http://hdl.handle.net/10125/7052 The objectives of this thesis research are as follows: 1. Perform nutritional analysis and create a database of selected nutrients for all currently fed feedstuffs for the six species (Guenther's dik-dik, black rhinoceros, Asian elephant, slender-tailed meerkat, Francois' langur and Raggiana bird of paradise) selected for dietary evaluation. 2. Determine 7-day feed consumption and calculate nutrient intakes for these six species through the weighing of feedstuffs and orts. 3. Determine appropriate nutrient intake levels for these six species. 4. Evaluate current diets for nutrient deficiencies or abundances by comparing calculated nutrient intakes to published appropriate nutrient levels for the species. 5. Formulate new balanced rations using local browse, by-products and other available feeds, along with acceptable feedstuff substitutions. xiv, 266 leaves Mon, 01 Dec 2003 00:00:00 GMT http://hdl.handle.net/10125/7052 2003-12-01T00:00:00Z Wilkemeyer, Chandra A http://hdl.handle.net/10125/7047 The focus of this study was to determine the effect of dietary fat content on assimilation efficiency (AE) in harbor seals. Rate of passage (peak at 24h) and initial defecation times (mean of 13.0h) were initially measured in five harbor seals on a typical high fat herring diet. Five harbor seals were used to assess the intake and nutrient digestibility of both high- and low-fat herring diets during the last 72 h of a 4-week feeding trial. The AE was determined using chromic oxide (Cr2O3 at 0.3% of the DM; placed in gel capsules and inserted into the opercular cavity of multiple herring) as the inert marker of indicator technique. All diet and fecal samples were analyzed for dry matter, ash, crude protein, and crude fat content. Carbohydrate and energy components were then calculated. Initial defecation times were longer than those found in previous studies at 13.0 ± 7.5h. Initial defecation times were found to be negatively correlated (r2= 0.85) to the total number of defecations. Percent Cr2O3 recovery was high at 85.33 ± 11.36%. Recovery of chromic oxide over 48 hours followed the expected bell-shaped curve with peak Cr2O3 recovery at ~24h after feeding. Assimilation efficiency of dry matter was significantly higher (p=0.0006) for the high fat herring, but AE's of crude protein, crude fat, and energy were similar between the two diets. This study confirms that fat content of herring does not affect nutrient AE's significantly when fed to harbor seals, but does have a significant (p=6.8*10-5 and 3.15*10-14) impact on the quantity of protein and fat consumed daily. The similarity of AE values of the high and low fat herring found in this study suggests that AE may be more dependent on prey species than was previously thought, and less dependent on the varying nutrient composition of any particular type (or kind) of prey. Further research should be conducted during both the cold and warm seasons to assess differences and determine relationships between level, type and proportion of nutrient intake on AE and animal energetic efficiency. These studies should mimic the nutritional circumstances in the wild in order to gain the best understanding of a possible relationship between nutrition and the decline of harbor seals populations. viii, 109 leaves Mon, 01 Dec 2003 00:00:00 GMT http://hdl.handle.net/10125/7047 2003-12-01T00:00:00Z Stanberry, Kathryn http://hdl.handle.net/10125/7041 Myostatin is a new member of the transforming growth factor-beta superfamily of secreted growth and differentiation factors. Myostatin is almost exclusively expressed in skeletal muscle and act as a negative regulator of skeletal muscle growth. Because anti-myostatin antibodies will be useful in investigating the mechanism of action of myostatin, an experiment was designed to produce polyclonal anti-chicken-myostatin antibodies. A PCR amplified prepro-myostatin composed of the prodomain plus mature myostatin and a C- terminal fragment containing only the mature myostatin were cloned into an expression vector, and these proteins were expressed in E. coli. The recombinant proteins were fractionated by SDS-PAGE, then the myostatin bands were cut out and electro-eluted to obtain purified myostatins. Rabbits were immunized with the purified myostatins to produce polyclonal anti-myostatin antibodies. IgGs were separated from the rabbit sera using Protein A affinity chromatography. Antibody binding characteristics were then examined using Western blot analysis of various chicken tissues. Both polyclonal antibodies demonstrated a strong affinity to both form of recombinant myostatin in Western blot analysis. The anti-mature myostatin antibody showed binding affinity to proteins at 50, 30 and 20 kD in skeletal muscle and liver. No specific binding in skeletal muscle was demonstrated by the anti-mature myostatin antibody, suggesting that the above proteins are non-myostatin proteins that have affinity to anti-mature myostatin antibody. In contrast, the anti-prepro-myostatin antibody showed affinity to a 37 kD band only in skeletal muscle in addition to the 50, 30 and 20 kD bands. Since the molecular weight of the myostatin prodomain is known to be close to 37 kD, it is postulated that the 37 kD protein specifically recognized by the antiprepro- myostatin in skeletal muscles is likely to be a myostatin prodomain with further validation of its binding specificity, the anti-prepro-myostatin antibody generated in this study will potentially be useful in investigating the mechanism of action of myostatin. x, 101 leaves Mon, 01 Dec 2003 00:00:00 GMT http://hdl.handle.net/10125/7041 2003-12-01T00:00:00Z Lee, Yun-Kyung http://hdl.handle.net/10125/7021 Black-footed albatross (Phoebastria nigripes) are among the many species of seabirds whose numbers are affected every year by anthropogenic impacts. Until now, no one has looked at the transfer of contaminants via soil to P. nigripes. However, studies have been done on how the Laysan albatross (P. immutabilis) is affected by lead in the soil (Work and Smith, 1996; Finkelstein et aI., 2003). Studies on endocrine disruption and effects of PCB contamination in wildlife have shown that estradiol and testosterone levels may be ahered and skewed as a result of anthropogenic sources (Fry and Toone, 1981; Grasman et aI., 1996; Auman et aI., 1997). Thus, the levels of gonadal steroids may function as indicators of exposure to polycWorinated biphenyls in the soil affecting these long-lived seabirds. The objective of this study was to determine if nesting in PCB-contaminated areas affects hormone balance due to endocrine disruption in both adult and chick black-footed albatross on Midway Atoll National Wildlife Refuge (NWR) and black-footed albatross chicks on Tern Island, Hawaiian Islands National Wildlife Refuge (HINWR). This thesis is divided into four parts. Chapter 1 contains the literature review and information on research background. The results for each island are presented in following chapters with the methods section similar at both locations. Chapter 2 compares hormone variation and soil contamination in two nesting sites on Midway Atoll. Chapter 3 discusses hormone variation between two nesting sites on Tern Island. Chapter 4 summarizes the work done in this study and suggests research that might be done in the future. viii, 73 leaves Mon, 01 Dec 2003 00:00:00 GMT http://hdl.handle.net/10125/7021 2003-12-01T00:00:00Z Bourdon, Kater Alisha